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Conditions of optimal MDH1 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Fax: 1-617-606-5019
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Introduction
Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.
BP000579-ENZ-598: Recombinant Malate Dehydrogenase Cytoplasmic (MDH1) Enzyme
Source: E. coli-derived.
A single polypeptide chain containing 336 amino acids (1-312) and having a molecular mass of 34.9 kDa. MDH is fused to a 24 amino acid His-tag at N-terminus.
Purity: > 95% by SDS-PAGE.
Formulation: The MDH solution (1 ug/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 50 mM Nacl, 1 mM DTT and 10% glycerol.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other MDH1 Enzymes:
Recombinant Human MDH1 Enzyme
Recombinant Human MDH1 Enzyme
Recombinant Chicken MDH1 Enzyme
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