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IL-10 Protein

Interleukin-10, Cytokine Synthesis Inhibitory Factor, CSIF, Il10, Il-10

Catalog No. Product Name Size List Price (US$) Quantity
BP000133-C027 Recombinant Human IL-10 Protein 10 ug 180.00
BP000133-C027 Recombinant Human IL-10 Protein 50 ug 528.00
BP000133-C027 Recombinant Human IL-10 Protein 1 mg 3660.00
Description

Introduction
Interleukin-10 (IL10) is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice is Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce IL10. In humans IL10 is produced by activated CD8 (+) peripheral blood T-cells, by T-helper CD4 (+) T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells.

BP000133-C027: Recombinant Human Interleukin-10 (IL-10) Protein

Source: E. coli-derived.
Recombinant Human Interleukin-10 (IL-10) Protein is a single non-glycosylated polypeptide chain containing 160 amino acids with a molecular mass of 18,648 Daltons.
N-terminal sequence analysis: Ser-Pro-Gly-Gln-Thr.
Predicted molecular mass: 18648 Dalton.
The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2 ng/ml, corresponding to a Specific Activity of 1.5 x 106 IU/mg.
Purity: > 95% by RP-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 300 mM NaCl, pH 7.3.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

Other IL-10 Proteins:
Recombinant Mouse IL-10 Protein
Recombinant Rat IL-10 Protein
Recombinant Porcine IL-10 Protein
Recombinant Mouse IL-10 Protein
Recombinant Human IL-10 Protein, His
Recombinant Human IL-10 Protein, HEK
Recombinant Rat IL-10 Protein
Recombinant Human IL-10 Protein

IL-10 Antibodies:
IL-10 Polyclonal Antibody
IL-10 Polyclonal Antibody
IL-10 Polyclonal Antibody
IL-10 Polyclonal Antibody
IL-10 Monoclonal Antibody
IL-10 Monoclonal Antibody

IL-10 ELISA Kits:
Rat IL-10 ELISA Kit
Mouse IL-10 ELISA Kit
Human IL-10 ELISA Kit

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