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Human Siglec-4a Protein

MAG; Siglec4a; Siglec-4a; S-MAG; GMA

Catalog No. Product Name Size List Price (US$) Quantity
BP004584 Human Siglec-4a/MAG Protein with the C-His Tag 100UG 270.00
BP004584 Human Siglec-4a/MAG Protein with the C-His Tag 500UG 900.00
BP005458 Human Siglec-4a/MAG Protein with the C-hFc Tag 100UG 270.00
BP005458 Human Siglec-4a/MAG Protein with the C-hFc Tag 500UG 900.00
Description

BP004584: Human Siglec-4a/MAG Protein with the C-His Tag

Source: The recombinant Human Siglec-4a/MAG Protein, (amino acids Gly20-Pro516) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Siglec-4a/MAG protein with the His tag at the C-terminus has a predicted MW of 55.8 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Siglec-4a protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Siglec-4a Antibody protein with the C-hFc tag with the EC50 of 4.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Siglec-4a/MAG Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005458: Human Siglec-4a/MAG Protein with the C-hFc Tag

Source: The recombinant Human Siglec-4a/MAG Protein, (amino acids Gly20-Pro516) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Siglec-4a/MAG protein with the hFc tag at the C-terminus has a predicted MW of 81.4 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Siglec-4a protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-Siglec-4a Antibody protein with the C-hFc tag with the EC50 of 13.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Adiponectin/Acrp30 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human Adiponectin/Acrp30 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following Siglec-4a Protein :
Biotinylated Human Siglec-4a Protein
Human Siglec-4a Protein
Mouse Siglec-4a Protein

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