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EK000145-EK0827: Human Rage ELISA Kit
Range 78pg/ml-5000pg/ml
Sensitivity < 10pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human Rage in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as ?GER? AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-?B).
Principle
The human Rage ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Rage specific-specific monoclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Rage amount of sample captured in plate.
Reference
1. Neeper M, Schmidt AM, Brett J, Yan SD, Wang F, Pan YC, Elliston K, Stern D, Shaw A (July 1992). "Cloning and expression of a cell surface receptor for advanced glycosylation end products of proteins". J. Biol. Chem. 267 (21): 14998?004. PMID1378843. 2. Bierhaus, A. Haslbeck, K.-M., Humpert, P. M., Liliensiek, B., Dehmer, T., Morcos, M., Sayed, A. A. R., Andrassy, M., Schiekofer, S., Schneider, J. G., Schulz, J. B., Heuss, D., and 12 others. Loss of pain perception in diabetes is dependent on a receptor of the immunoglobulin superfamily. J. Clin. Invest. 114: 1741-1751, 2004. [PubMed: 15599399, related citations] [Full Text: Journal of Clinical Investigation, Pubget]
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