Human MSLN Protein

BP003149: Human MSLN/Mesothelin Protein with the C-His Tag

Source: The recombinant Human MSLN/Mesothelin Protein, (amino acids Glu296-Ser598) fused with a His tag at the C-terminus, was produced in HEK293 cells.
Molecular Weight: The recombinant Human MSLN/Mesothelin protein with the His tag at the C-terminus has a predicted MW of 35.23 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human MSLN protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-MSLN Antibody protein with the C-hFc tag with the EC50 of 2.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human MSLN/Mesothelin Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP003115: Human MSLN/Mesothelin Protein with the C-His Tag

Source: The recombinant Human MSLN/Mesothelin Protein, (amino acids Glu296-Leu591) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human MSLN/Mesothelin protein with the His tag at the C-terminus has a predicted MW of 34.48 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human MSLN protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-MSLN Antibody protein with the C-hFc tag with the EC50 of 2.6 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human MSLN/Mesothelin Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP003125: Human MSLN/Mesothelin Protein with the C-hFc Tag

Source: The recombinant Human MSLN/Mesothelin Protein, (amino acids Ala488-Ser600) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human MSLN/Mesothelin protein with the hFc tag at the C-terminus has a predicted MW of 39.30 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human MSLN protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-MSLN Antibody protein with the C-hFc tag with the EC50 of 15.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human MSLN/Mesothelin Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005455: Human MSLN/Mesothelin Protein with the C-hFc Tag

Source: The recombinant Human MSLN/Mesothelin Protein, (amino acids Glu296-Gly580) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human MSLN/Mesothelin protein with the hFc tag at the C-terminus has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human MSLN protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-MSLN Antibody protein with the C-hFc tag with the EC50 of 44.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human MSLN/Mesothelin Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005311: Human MSLN/Mesothelin Protein with the C-His-Avi Tag

Source: The recombinant Human MSLN/Mesothelin Protein, (amino acids Glu296-Gly580) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human MSLN/Mesothelin protein with the His-Avi tag at the C-terminus has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human MSLN protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well). Dose response curve for the recombinant Anti-MSLN Antibody protein with the C-hFc tag with the EC50 of 12.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human MSLN/Mesothelin Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

Shipping: The recombinant Human MSLN/Mesothelin Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following MSLN Protein :
Biotinylated Cynomolgus MSLN Protein
Biotinylated Human MSLN Protein
Biotinylated Mouse MSLN Protein
Cynomolgus MSLN Protein
FITC-Labeled Human MSLN Protein
Human MSLN Protein
Mouse MSLN Protein
PE-Labeled Human MSLN Protein