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Conditions of optimal recombinant Human Complement C5 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Fax: 1-617-606-5019
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BP003886: Human Complement C5 Protein with the C-His Tag
Source: The recombinant Human Complement C5 Protein, (amino acids Gln19-Cys1676) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Complement C5 protein with the His tag at the C-terminus is cleaved into α chain and β chain by the enzyme lysis. Each chain has a predicted MW of 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Complement C5 protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Complement C5 Antibody protein with the C-hFc tag with the EC50 of 15.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Complement C5 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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