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EK000146-EK0706: Human CD56/NCAM-1 ELISA Kit
Range 78pg/ml-5000pg/ml
Sensitivity < 10pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human CD56 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C
Background
Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
Principle
The human CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human CD56 specific-specific monoclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD56 amount of sample captured in plate.
Reference
1. Reyes AA, Small SJ, Akeson R. (1991). "At least 27 alternatively spliced forms of the neural cell adhesion molecule mRNA are expressed during rat heart development". Mol Cell Biol. 11 (3): 1654?1. PMC 369464. PMID 1996115.
2. Nguyen, C., Mattei, M. G., Mattei, J.-F., Santoni, M.-J., Goridis, C., Jordan, B. R. Localization of the human NCAM gene to band q23 of chromosome 11: the third gene coding for a cell interaction molecule mapped to the distal portion of the long arm of chromosome 11. J. Cell Biol. 102: 711-715, 1986. [PubMed: 2869046, related citations] [Full Text: HighWire Press, Pubget].
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