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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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BP003485: Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-His Tag
Source: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein, (amino acids Ile20-Asp503) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG protein with the His tag at the C-terminus has a predicted MW of 53.67 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Alkaline Phosphatase (Germ type) protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Alkaline Phosphatase Antibody protein with the C-hFc tag with the EC50 of 6.6 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP003375: Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-hFc Tag
Source: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein, (amino acids Ile20-Asp503) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG protein with the hFc tag at the C-terminus has a predicted MW of 79.33 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Alkaline Phosphatase (Germ Type) protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-Alkaline Phosphatase Antibody protein with the C-hFc tag with the EC50 of 15.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP003374: Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-mFc Tag
Source: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein, (amino acids Ile20-Asp503) fused with a mFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG protein with the mFc tag at the C-terminus has a predicted MW of 78.24 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Alkaline Phosphatase (Germ type)rotein with the C-mFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Alkaline Phosphatase Antibody protein with the C-hFc tag with the EC50 of 29.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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