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Human Alkaline Phosphatase (Germ type) Protein

Catalog No. Product Name Size List Price (US$) Quantity
BP003485 Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-His Tag 100UG 315.00
BP003485 Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-His Tag 500UG 1050.00
BP003375 Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-hFc Tag 100UG 315.00
BP003375 Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-hFc Tag 500UG 1050.00
BP003374 Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-mFc Tag 100UG 315.00
BP003374 Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-mFc Tag 500UG 1050.00
Description

BP003485: Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-His Tag

Source: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein, (amino acids Ile20-Asp503) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG protein with the His tag at the C-terminus has a predicted MW of 53.67 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Alkaline Phosphatase (Germ type) protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Alkaline Phosphatase Antibody protein with the C-hFc tag with the EC50 of 6.6 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP003375: Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-hFc Tag

Source: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein, (amino acids Ile20-Asp503) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG protein with the hFc tag at the C-terminus has a predicted MW of 79.33 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Alkaline Phosphatase (Germ Type) protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-Alkaline Phosphatase Antibody protein with the C-hFc tag with the EC50 of 15.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP003374: Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-mFc Tag

Source: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein, (amino acids Ile20-Asp503) fused with a mFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG protein with the mFc tag at the C-terminus has a predicted MW of 78.24 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Alkaline Phosphatase (Germ type)rotein with the C-mFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Alkaline Phosphatase Antibody protein with the C-hFc tag with the EC50 of 29.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following Alkaline Phosphatase (Germ type) Protein :
Biotinylated Human Alkaline Phosphatase (Germ type) Protein
Human Alkaline Phosphatase (Germ type) Protein
Mouse Alkaline Phosphatase (Germ type) Protein
Rat Alkaline Phosphatase (Germ type) Protein

Syd Labs provides the following Alkaline Phosphatase (Placental type) Protein :
Cynomolgus Alkaline Phosphatase (Placental type) Protein
Human Alkaline Phosphatase (Placental type) Protein

Syd Labs provides the following Alkaline phosphatase (Intestinal type) Protein :
Human Alkaline phosphatase (Intestinal type) Protein
Mouse Alkaline phosphatase (Intestinal type) Protein

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