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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Conditions of optimal HBV Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
BP000416-HBV-271: Recombinant HBV Protein X
Source: E. coli-derived.
Predicted molecular mass: 17 kDa.
Purity > 98%, by SDS-PAGE.
Formulation: Filtered (0.4 um) and lyophilized from 0.5 ug/ml in 0.05 M phosphate buffer, 0.075 M NaCl, pH7.4.
Reconstitution: It is recommended to add deionized water to prepare a working stock solution of 0.5 ug/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications: WB, ELISA.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other HBV Proteins:
Recombinant HBV HBe
Recombinant HBV HBe
Recombinant HBV Core delta
Recombinant HBV Core (1-183 a.a.)
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