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Conditions of optimal GRP94 antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
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Introduction
HSP90B1 is an abundant molecular chaperone resident endoplasmic reticulum (ER) lumenal stress protein which is part of the Hsp90 family. HSP90B1 is involved in maintaining protein homeostasis in the secretory pathway as well as functioning in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. HSP90B1 has key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90B1 protein associates with numerous cochaperones and involved in folding of newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is highly expressed in human gastric carcinoma BGC-823 cells during the whole cell cycle.
PA001896-ANT-325: HSP90B1 (GRP94) Monoclonal Antibody
Mouse Monoclonal Antibody.
Applications: HSP90B1 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Immunofluorescence analysis is 1:100 ~ 1:200. Recommended starting dilution is 1:100.
Reactivity: Human.
Immunogen: Anti-human HSP90B1 mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HSP90B1 amino acids 676-803 purified from E. coli.
Purification: HSP90B1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form of Antibody: 1 ug/ml containing PBS, pH-7.4, & 0.1% Sodium Azide
Clone: P2H3AT.
Isotype: Mouse IgG2a.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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