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Conditions of optimal eIF4E antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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Introduction
Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the eIF4E gene. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methyl-guanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
PA001545-B7067: eIF4E (Ab-209) Polyclonal Antibody
Rabbit Polyclonal Antibody.
Specificity/Sensitivity: elF4E (Ab-209) antibody detects endogenous lev els of total elF4E protein.
Applications: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000.
Reactivity: Human, Mouse, Rat.
Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+0.02% sodium azide and 50% glycerol.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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