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EK000060-EK0425: Mouse IL-13 ELISA Kit
Range 15.6pg/ml-1000pg/ml
Sensitivity < 2 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitive detection of mouse IL-13 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Interleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases.1 Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses.2 IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy.3 The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5KDa.
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Fichtner-Feigl, S.; Strober, W.; Kawakami, K.; Puri, R. K.; Kitani, A. IL-13 signaling through the IL-13-alpha-2 receptor is involved in induction of TGF-beta-1 production and fibrosis. Nature Med. 12: 99-106, 2006.
2. Minty, A.; Chalon, P.; Derocq, J.-M.; Dumont, X.; Guillemot, J.-C.; Kaghad, M.; Labit, C.; Leplatois, P.; Liauzun, P.; Miloux, B.; Minty, C.; Casellas, P.; Loison, G.; Lupker, J.; Shire, D.; Ferrara, P.; Caput, D. Interleukin-13 is a new human lymphokine regulating inflammatory and immune responses. Nature 362: 248-250, 1993.
3. Kelly-Welch, A. E.; Hanson, E. M.; Boothby, M. R.; Keegan, A. D. Interleukin-4 and interleukin-13 signaling connections maps. Science 300: 1527-1528, 2003.
4. Smirnov, D. V.; Smirnova, M. G.; Korobko, V. G.; Frolova, E. I. Tandem arrangement of human genes for interleukin-4 and interleukin-13: resemblance in their organization. Gene 155: 277-281, 1995.
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