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Conditions of optimal DanK Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak(residues 508-638) of the substrate binding domain is a-helical and appears to act as a lid covering the substrate binding cleft. DnaK(amino acid 508-638) was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus.
BP001983-HSP-009: Recombinant E.Coli Dnak Substrate Binding Domain C-terminal (DnaK) Protein
Source: E. coli-derived.
Recombinant Dnak Substrate Binding Domain C-terminal protein is a single, non-glycosylated polypeptide chain containing 255 amino acids and having a molecular mass of 27.7 kDa.
Purity: > 95.0% as determined by (a) Analysis by RP-HPLC; (b) Analysis by SDS-PAGE.
Formulation: The DnaK protein contains 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5 mM DTT and 10% Glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other DnaK Proteins:
Recombinant Human DnaK, His Tag Protein
Recombinant E.Coli DnaK Substrate Binding Domain Protein
Recombinant E.Coli DnaK Lid Covering Substrate Protein
Recombinant E.Coli DnaK (HSP70) Protein
Recombinant Mycobacterium Tuberculosis DnaK (HSP70) Protein
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