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Conditions of optimal DMT-2'-O-TBDMS-A(Bz)-CE-Phosphoramidite performance should be determined experimentally by the investigator. Its precursors, N6-Bz-rA (CAS # 4546-55-8) and N6-Bz-2'-O-TBDMS-5'-O-DMT-A (CAS # 81265-93-2), are available.
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APIM050508: DMT-2'-O-TBDMS-A(Bz)-CE-Phosphoramidite
CAS No.: 104992-55-4.
Molecular Formula: C53H66N7O8PSi.
Molecular Weight: 988.19.
Appearance: (visual inspection) white to off-white powder.
Identity: (molecular weight by MS) conforms to structure molecular weight; (1H NMR) conforms to structure.
Purity: (31P NMR) ≥99.0%; (RP-HPLC) ≥99.0%.
Impurity profile: (HPLC) any single critical impurity ≤ 0.15%; total critical impurities ≤ 0.3%; total impurities ≤ 1.0%.
Assay: (HPLC) 98.0%~102.0%.
Sulfate ash: (muffle furnace) ≤0.1%.
Residual solven: (GC) ≤1.5%.
Moisture content: (Karl Fischer) ≤0.2%
Clarity of solution: (visual inspection) the solution shall be clear and free of insoluble substances.
Storage: -20±5℃.
2-TBDMS-Phosphoramidites:
DMT-2'-O-TBDMS-A(Bz)-CE-Phosphoramidite (CAS No.: 104992-55-4)
DMT-2'-O-TBDMS-G(iBu)-CE-Phosphoramidite (CAS No.: 147201-04-5)
DMT-2'-O-TBDMS-C(Ac)-CE-Phosphoramidite (CAS No.: 121058-88-6)
DMT-2'-O-TBDMS-U-CE-Phosphoramidite (CAS No.: 118362-03-1)
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