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Conditions of optimal DDX5/DEAD-box Protein 5 antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.
PA001422-B0903: DDX5/DEAD-box Protein 5 (Ab-593) Polyclonal Antibody
Rabbit Polyclonal Antibody.
Specificity/Sensitivity: DDX5/DEAD-box Protein 5 (Phospho-Tyr593) antibody detects endogenous levels of DDX5/DEAD-box Protein 5 only when phosphorylated at tyrosine 593.
Applications: IF: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000.
Reactivity: Human, Mouse, Rat.
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human DDX5/DEAD-box Protein 5 around the phosphorylation site of tyrosine 593 (Q-A-YP-A-Y).
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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