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Conditions of optimal COT (Phosphorylated) antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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PA001295-A0064: COT (Phospho-Thr290) Polyclonal Antibody
Rabbit Polyclonal Antibody.
Specificity/Sensitivity: COT (phospho-Thr290) antibody detects endogenous levels of COT only when phosphorylated at threonine 290.
Applications: WB: 1:300~1:1000 IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:40000.
Reactivity: Human, Mouse, Rat.
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human COT around the phosphorylation site of threonine 290 (R-G-TP-E-I).
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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