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Conditions of optimal cofilin antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Introduction
ADF/cofilin is a family of actin-binding proteins which disassembles actin filaments. Actin-binding proteins regulate assembly and disassembly of actin filaments. Cofilin, a member of the ADF/cofilin family is actually a protein with 70% sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. The protein binds to actin monomers and filaments, G actin and F actin, respectively. Cofilin causes depolymerization at the minus end of filaments, thereby preventing their reassembly. The protein is known to sever actin filaments by creating more positive ends on filament fragments. Cofilin/ADF(destrin) is likely to sever F-actin without capping and prefers ADP-actin.
PA000385-R12-2640: Cofilin Polyclonal Antibody
Rabbit Polyclonal Antibody.
Specificity/Sensitivity: Cofilin antibody detects endogenous levels of Cofilin.
Applications: WB: 1:500~1:1000; ELISA: 1:10000.
Reactivity: Human, Mouse, Rat.
Immunogen: The antiserum was produced against synthesized peptide derived from human Cofilin.
Purification: The antibody was affinity-purified from rabbit anti-serum by immunogenic peptide affinity-chromatography.
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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