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Conditions of optimal CENPB Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.
CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome.
BP001880-PRO-390: Recombinant Human Centromere Protein B (CENPB)
Source: Sf9 insect cells-derived.
Recombinant Human CENPB is a glycosylated, polypeptide chain having a molecular mass of 65,940 Dalton. CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Immunological functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Purity: > 90% as determined by SDS-PAGE.
Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Formulation: CENPB is supplied in 20 mM HEPES buffer pH-7.6, 250 mM sodium chloride, and 20% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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