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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal CDC42 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
CDC42 (Cell division cycle 42 isoform 1) is a small GTPase of the Rho-subfamily that regulates signaling pathways which control various cellular functions including cell morphology, migration, endocytosis and cell cycle progression. CDC42 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In the active state, CDC42 binds to a variety of effector proteins to regulate cellular responses. CDC42 is involved in epithelial cell polarization processes. CDC42 causes the formation of thin, actin-rich surface projections called filopodia. Also, CDC42 regulates actin polymerization through its direct binding to N-WASP (Neural Wiskott-Aldrich syndrome protein), which subsequently activates Arp2/3 complex.
Loss of CDC42 function raises endocytotic uptake of apical proteins, as well as apical polarity factors such as Crumbs. The product of oncogene Dbl was described to specifically catalyze the dissociation of GDP from CDC42 protein.
BP001874-PRO-727: Recombinant Human Cell Division Cycle 42 (CDC42) Protein
Source: E. coli-derived.
Recombinant Human CDC42 fused with a 15 amino acid T7 tag at N-terminus is a single, non-glycosylated, polypeptide chain containing 203 amino acids (1-188 a.a.) and having a molecular mass of 22.4 kDa. The CDC42 is purified by proprietary chromatographic techniques.
Purity: > 95.0% as determined by SDS-PAGE.
Formulation: The CDC42 solution contains 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol and 2 mM EDTA.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
CDC42 Antibodies:
CDC42 Polyclonal Antibody
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