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Conditions of optimal recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein performance should be determined experimentally by the investigator.
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Phone: 1-617-401-8149
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BP004271: Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein with the C-His-Avi Tag
Source: The recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein, (amino acids Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer protein with the His-Avi tag at the C-terminus has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody protein with the C-hFc tag with the EC50 of 3.9 ng/ml determined by ELISA (QC Test). The affinity constant of 0.111 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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