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EK000111-EK0644: Human CD105 ELISA Kit
Range 156ug/ml-10,000ug/ml
Sensitivity < 5 ug/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human CD105 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Rius, C.; Smith, J. D.; Almendro, N.; Langa, C.; Botella, L. M.; Marchuk, D. A.; Vary, C. P. H.; Bernabeu, C. : Cloning of the promoter region of human endoglin, the target gene for hereditary hemorrhagic telangiectasia type 1. Blood 92: 4677-4690, 1998.
2. McAllister, K. A.; Grogg, K. M.; Johnson, D. W.; Gallione, C. J.; Baldwin, M. A.; Jackson, C. E.; Helmbold, E. A.; Markel, D. S.; McKinnon, W. C.; Murrell, J.; McCormick, M. K.; Pericak-Vance, M. A.; Heutink, P.; Oostra, B. A.; Haitjema, T.; Westerman, C. J. J.; Porteous, M. E.; Guttmacher, A. E.; Letarte, M.; Marchuk, D. A. : Endoglin, a TGF-beta binding protein of endothelial cells, is the gene for hereditary haemorrhagic telangiectasia type 1. Nature Genet. 8: 345-351, 1994.
3. Lebrin, F.; Goumans, M.-J.; Jonker, L.; Carvalho, R. L. C.; Valdimarsdottir, G.; Thorikay, M.; Mummery, C.; Arthur, H. M.; ten Dijke, P. : Endoglin promotes endothelial cell proliferation and TGF-beta/ALK1 signal transduction. EMBO J. 23: 4018-4028, 2004.
4. Grisanti, S.; Canbek, S.; Kaiserling, E.; Adam, A.; Lafaut, B.; Gelisken, F.; Szurman, P.; Henke-Fahle, S.; Oficjalska-Mlynczak, J.; Bartz-Schmidt, K. U. : Expression of endoglin in choroidal neovascularization. Exp. Eye Res. 78: 207-213, 2004.
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