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Conditions of optimal CaMKII (Phosphorylated) antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
CaMKII is one of the most abundant proteins in neurons making 1-2% of the total protein concentration. CaMKII forms heterooligomers of 12 subunits and arrange in a hexameric doubledoughnut structure. There are now > 30 isoforms of CaMKII known, comprised of alternately spliced variants of alpha, beta, delta. Primary isoforms are alpha and beta. Depending on the location in the brain the ratio between the two forms can range from 3:1 = alpha:beta (forebrain mouse) to 1:4 (cerebellum). BetaCaMKII differs from the alpha form in the variable region for F-actin targeting. It was reported that alpha and beta isoforms have sharply different affinities for CaM (Half-max autophos level is achieved at 130 nM CaM for alphaCaMKII and at 15 nM CaM for betaCaMKII). Heterooligomers comprised of two beta subunits translocate to the postsynaptic density (PSD) because of the F-actin targeting of betaCaMKII. At its site Thr286 CaMKII can be phosed by a particular binding event with CaM (detailed below). When phosed on T286 CaMKII can trap bound CaM by a several hundred fold increase in binding affinity. CaMKII (also alphaCaMKII) translocates to the PSD in response to NMDA receptor activation.
PA000398-A7033: CaMK2 (Phospho-Thr286) Polyclonal Antibody
Rabbit Polyclonal Antibody.
Specificity/Sensitivity: CaMK2 (Phospho-Thr286) antibody detects endogenous levels of CaMK2 only when phosphorylated at threonine 286.
Applications: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:40000.
Reactivity: Human, Mouse, Rat.
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human CaMK2 around the phosphorylation site of threonine 286 (Q-E-TP-V-D).
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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