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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal APAF1 antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Background
Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23.1 It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis.2 This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9.3 Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death.4 Additionally, APAF1 has been shown to interact with NLRP1,5 Caspase-9,5, 6, 7, 8, 9, 10 APIP,6 BCL2-like 18, 9 and HSPA4.10
PA000266-PA1249-1: APAF1 Polyclonal Antibody
Rabbit Polyclonal Antibody.
Specificity/Sensitivity: Human, rat, mouse. No cross reactivity with other proteins.
Applications: WB: 0.1-0.5 ug/ml IHC-P: 0.5-1 ug/ml.
Reactivity: Human, Mouse, Rat.
Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human APAF1, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Purification: Immunogen affinity purified.
Form of Antibody: Lyophilized. Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Isotype: Rabbit IgG.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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