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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 10A7.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse TIGIT monoclonal antibody (clone: 10A7) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10A7 antibody specifically binds to mouse TIGIT protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 10A7.
Isotype: Human IgG1 kappa.
Source: The anti-mouse TIGIT monoclonal antibody (clone: 10A7) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10A7 antibody specifically binds to mouse TIGIT protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 10A7 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Background
The 10A7 antibody binds to the TIGIT protein (WUCAM, Vstm3 or VSIG9), a novel immune checkpoint receptor with inhibitory function. TIGIT is expressed on T cells and natural killer (NK) cells, and several human cancers, including melanoma, NSCLC, and colorectal cancer. Similar to PD-1, the TIGIT receptor limits antitumor immune response in cancer.
Integrative analysis of neuroblastoma by single-cell RNA sequencing identifies the NRP2+ neuroblastic progenitor cell state as a novel target for immunotherapy.
Zeng W, et al. Cancer Cell. 2024 Feb 12;42(2):226-240.e8. doi: 10.1016/j.ccell.2023.12.002. PMID: 38168431
To determine whether the NRP2+ neuroblastic progenitor state could be targeted for immunotherapy, we combined anti-TIGIT antibody clone 10A7 with anti-PD-L1 in syngeneic neuroblastoma mouse models. Single-cell suspensions were stained with fluorochrome-conjugated antibodies for flow cytometry. Tumor growth was monitored by caliper measurements twice weekly. The combination therapy significantly reduced tumor burden compared to controls. Flow cytometry data were analyzed using FlowJo software.
Tags: function of 10A7 antibody; 10A7 antibody for animal model
The immune checkpoint TIGIT is upregulated on T cells during infection and downregulates T cell function to limit antibacterial immunity.
Lau L, et al. Front Immunol. 2023;14:1238554. doi: 10.3389/fimmu.2023.1238554. PMID: 37604048
Anti-TIGIT antibody (clone 10A7) was administered intraperitoneally to block TIGIT during Salmonella infection. Flow cytometry confirmed upregulation of TIGIT on CD8+ T cells using clone 10A7 (anti-mouse TIGIT antibody). Infected mice were monitored for bacterial burden in spleen and liver. The blockade enhanced antibacterial T cell responses. Data were acquired on a BD LSRFortessa flow cytometer.
Tags: 10A7 antibody for flow cytometry; 10A7 mAb for flow cytometry
Enhanced antitumor efficacy of a novel oncolytic vaccinia virus encoding a fully monoclonal antibody against TIGIT.
Zuo S, et al. EBioMedicine. 2021 Jul;69:103441. doi: 10.1016/j.ebiom.2021.103441. PMID: 34120188
The oncolytic vaccinia virus was engineered to express a single-chain variable fragment (scFv) derived from the anti-TIGIT antibody clone 10A7. In vivo studies were conducted in BALB/c mice bearing CT26 tumors. Tumor volumes were measured every 3 days post-treatment. The engineered virus significantly prolonged survival compared to control virus. Flow cytometry confirmed increased CD8+ T cell infiltration in treated tumors.
Tags: anti-mouse TIGIT 10A7 of low endotoxin; anti-mouse TIGIT 10A7
An engineered oncolytic vaccinia virus encoding a fully monoclonal antibody against TIGIT induces effective antitumor responses through increased M1 macrophage recruitment.
Wei J, et al. J Immunother Cancer. 2023 Aug;11(8):e007025. doi: 10.1136/jitc-2023-007025. PMID: 37604048
The virus encodes the heavy and light chains of anti-TIGIT antibody (clone 10A7) connected by a self-cleaving 2A peptide. Tumor-bearing mice were treated intratumorally with the engineered virus. Flow cytometry was used to analyze macrophage polarization using anti-F4/80 and anti-CD86. The treatment increased M1 macrophage recruitment in the tumor microenvironment. Survival was significantly improved in the treatment group.
Tags: activity of anti-mouse TIGIT; anti-mouse TIGIT (10A7)
Structure of TIGIT immunoreceptor bound to poliovirus receptor reveals a cell-cell adhesion cis-trans regulatory mechanism.
Stengel KF, et al. Proc Natl Acad Sci U S A. 2012 Mar 20;109(14):5399-5404. doi: 10.1073/pnas.1120606109. PMID: 22423183
The anti-TIGIT antibody 10A7 was used to stain human T cells for flow cytometry to assess TIGIT expression. Crystal structures were determined for TIGIT bound to PVR. In vivo blocking experiments with 10A7 (anti-mouse TIGIT mAb) enhanced T cell responses in mouse models. Flow cytometry data showed 10A7 (anti-mouse TIGIT antibody) binding to TIGIT-transfected cells. The cis-trans regulatory mechanism was validated in cell adhesion assays.
Tags: activity of anti-mouse TIGIT; anti-mouse TIGIT 10A7 antibody
For more references about anti-mouse TIGIT antibody (10A7), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse TIGIT monoclonal antibodies:
Recombinant Anti-mouse TIGIT monoclonal antibodies (Clone: 1F4)
Recombinant Anti-mouse TIGIT monoclonal antibodies (Clone: 10A7)
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