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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 10A7.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse TIGIT monoclonal antibody (clone: 10A7) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10A7 antibody specifically binds to mouse TIGIT protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 10A7.
Isotype: Human IgG1 kappa.
Source: The anti-mouse TIGIT monoclonal antibody (clone: 10A7) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10A7 antibody specifically binds to mouse TIGIT protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 10A7.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse TIGIT monoclonal antibody (clone: 10A7) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 10A7 antibody specifically binds to mouse TIGIT protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 10A7 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Background
The 10A7 antibody binds to the TIGIT protein (WUCAM, Vstm3 or VSIG9), a novel immune checkpoint receptor with inhibitory function. TIGIT is expressed on T cells and natural killer (NK) cells, and several human cancers, including melanoma, NSCLC, and colorectal cancer. Similar to PD-1, the TIGIT receptor limits antitumor immune response in cancer.
Development of Novel Monoclonal Antibodies for Evaluation of Transmembrane Prostate Androgen-Induced Protein 1 (TMEPAI) Expression Patterns in Gastric Cancer.
Karbyshev, M. S. et al. Pathol Oncol Res. 2018 Apr;24(2):427-438. doi: 10.1007/s12253-017-0247-x. PMID: 28584893
Immunoblotting analysis showed that monoclonal antibodies, 2E1, 6C6, and 10A7 were able to recognize specifically target protein in transiently transfected HEK293T and CHO-K1 cells. ...Especially established mAb, named 10A7, showed the excellent binding ability to target protein in immunohistochemistry. ...By using developed antibodies, we observed pronounced expression of TMEPAI in normal gastric epithelial cells while tumor cells from gastric adenomas, and adenocarcinoma samples were mostly negative for target protein expression. ...Also, we found that gastric epithelium cells lose the TMEPAI expression concurrently with severe dysplasia progression, which probably caused by a mechanism involving specific microRNA. ...Transmembrane prostate androgen-induced protein 1 (TMEPAI) is a single-span membrane protein, functionally involved in transforming growth factor beta signaling pathway.
Tags: function of 10A7 antibody; 10A7 antibody for animal model
For more references about anti-mouse TIGIT antibody (10A7) please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse TIGIT monoclonal antibodies:
Recombinant Anti-mouse TIGIT monoclonal antibodies (Clone: 1F4)
Recombinant Anti-mouse TIGIT monoclonal antibodies (Clone: 10A7)
Syd Labs provides the following recombinant anti-mouse TIGIT monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse TIGIT monoclonal antibodies (Clone: 1F4) for flow cytometry
Recombinant Anti-mouse TIGIT monoclonal antibodies (Clone: 10A7) for flow cytometry
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