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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype control antibody are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Kappa Monoclonal Antibody.
Clone: MECA-32.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse PLVAP/PV-1 monoclonal antibody (clone: MECA-32) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: MECA-32) specifically binds to the mouse PLVAP/PV-1 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse PLVAP/PV-1 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The In vivo Grade Recombinant Anti-mouse PLVAP/PV-1 Monoclonal Antibody, Rat IgG2a Kappa (Clone: MECA-32) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Endothelial cell-derived angiopoietin-like protein 2 supports hematopoietic stem cell maintenance in the mouse bone marrow niche
Lin MI, et al. Stem Cells. 2022 Jan;40(1):80-91. doi: 10.1002/stem.3466. PMID: 34657340
Bone marrow sections were stained with MECA-32 antibody to identify endothelial cells in vivo. MECA-32+ ECs co-localized with Angptl2 in the BM niche. In vivo depletion of Angptl2 reduced HSC maintenance in the BM. MECA-32 staining confirmed vascular integrity in Angptl2-deficient mice. HSC frequency decreased in BM post-Angptl2 knockout.
Tags: anti-mouse PLVAP/PV-1 MECA-32; anti-mouse PLVAP/PV-1 MECA-32 antibody
VEGF-A modulates perivascular niche of human hematopoietic stem cells
Ding L, et al. Blood. 2018 Jun 7;131(23):2566-2576. doi: 10.1182/blood-2017-11-817171. PMID: 29754876
Vascular endothelial cells were labeled with MECA-32 antibody in vivo to assess VEGF-A effects. MECA-32+ vessels showed increased density post-VEGF-A administration. In vivo VEGF-A deletion disrupted perivascular HSC niches. HSC engraftment was impaired in VEGF-A-deficient BM. MECA-32 staining confirmed EC alterations in knockout mice.
Tags: anti-mouse PLVAP/PV-1 MECA-32 in vivo; anti-mouse PLVAP/PV-1 MECA-32 antibody in vivo
Endothelial cell-derived SLIT3 regulates intestinal stem cell regeneration
Aoki R, et al. J Clin Invest. 2017 Aug 1;127(8):3043-3056. doi: 10.1172/JCI93504. PMID: 28723562
Intestinal vasculature was stained with MECA-32 antibody to track ECs in vivo. MECA-32+ vessels supported ISC regeneration post-irradiation. In vivo SLIT3 deletion reduced vascular support for ISCs. MECA-32 staining revealed decreased vessel density in knockouts. ISC proliferation was impaired in SLIT3-deficient intestines.
Tags: anti-mouse PLVAP/PV-1 MECA-32 in animal model; anti-mouse PLVAP/PV-1 MECA-32 antibody in animal model
Endothelial-specific deletion of autophagy gene Atg7 suppresses tumorigenesis in a mouse model of liver cancer
Khambu B, et al. Hepatology. 2016 Jun;63(6):1837-49. doi: 10.1002/hep.28508. PMID: 26902621
Liver tumor vasculature was labeled with MECA-32 antibody for in vivo endothelial analysis. MECA-32+ ECs showed reduced density in Atg7-deficient tumors. In vivo Atg7 deletion suppressed tumor angiogenesis. Tumor growth was attenuated in endothelial-specific Atg7 knockouts. MECA-32 staining confirmed vascular defects in the tumor microenvironment.
Tags: anti-mouse PLVAP/PV-1 MECA-32 antibody in cancer research; anti-mouse PLVAP/PV-1 MECA-32 in mouse tumor model
Role of endothelial cells in early pancreas and liver development
Lammert E, et al. Mech Dev. 2013 Jan-Feb;130(1):44-50. doi: 10.1016/j.mod.2012.12.001. PMID: 23276718
Embryonic pancreatic tissues were stained with MECA-32 antibody to detect endothelial cells in vivo. MECA-32+ ECs were critical for early pancreatic bud formation. In vivo EC ablation disrupted liver and pancreas organogenesis. MECA-32 staining showed vascular networks in developing organs. Endothelial signals supported endocrine cell differentiation in vivo.
Tags: anti-mouse PLVAP/PV-1 antibody MECA-32; clone MECA-32 of anti-mouse PLVAP/PV-1 antibody
For more references about anti-mouse PLVAP/PV-1 antibody (Clone: MECA-32), please contact our scientific support team with message@sydlabs.com.
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