PA007627.r2b: In Vivo Grade Recombinant Anti-mouse Ly6G/Ly6C (Gr-1) Monoclonal Antibody (Clone: RB6-8C5), Rat IgG2b Kappa
Recombinant rat IgG2b Monoclonal Antibody.
Clone: RB6-8C5.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse Ly6G/Ly6C (Gr-1) monoclonal antibody (clone: RB6-8C5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: RB6-8C5) specifically binds to mouse Ly6G/Ly6C (Gr-1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse Ly6G/Ly6C (Gr-1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse Ly6G/Ly6C (Gr-1) monoclonal antibody of clone RB6-8C5 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse Ly6G/Ly6C (Gr-1) antibody (Clone: RB6-8C5):
Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice
Dalpke AH, et al. J Leukoc Biol. 2008 Jan;83(1):64-70. doi: 10.1182/jlb.0607372. PMID: 17884993
The anti-granulocyte receptor-1 (Gr-1) monoclonal antibody RB6-8C5, which binds to Ly6G on neutrophils and Ly6C on neutrophils, dendritic cells, and subpopulations of lymphocytes and monocytes, was used to deplete neutrophils in mice. In vivo administration of RB6-8C5 reduced blood neutrophils and Gr-1+ monocytes, while the Ly6G-specific mAb 1A8 selectively depleted neutrophils without affecting Gr-1+ monocytes, offering a more specific alternative for studying neutrophil roles in host defense.
Tags: anti-mouse Ly6G/Ly6C (Gr-1) RB6-8C5; anti-mouse Ly6G/Ly6C (Gr-1) (RB6-8C5) in vivo antibody
Ly6C+ Ly6G- Myeloid-derived suppressor cells play a critical role in the resolution of acute inflammation and the subsequent tissue repair process after spinal cord injury
Wang J, et al. J Neuroinflammation. 2013 Dec 30;10:154. doi: 10.1186/1742-2094-10-154. PMID: 23278273
The anti-Gr-1 antibody (clone RB6-8C5) was used to deplete Ly6C+ Ly6G- myeloid-derived suppressor cells (MDSCs) prior to spinal cord injury (SCI) in C57BL/6 mice, induced at T9-10 using a 1.0 mm impactor with 0.8 N force. Depletion exacerbated tissue edema, vessel permeability, and hemorrhage, worsening neurological outcomes. Intraspinal transplantation of ex vivo-generated MDSCs reduced inflammation and promoted tissue regeneration, highlighting the critical role of Ly6C+ Ly6G- cells in SCI recovery.
Tags: anti-mouse Ly6G/Ly6C (Gr-1) RB6-8C5 antibody; anti-mouse Ly6G/Ly6C (Gr-1) antibody (clone RB6-8C5)
Specific depletion reveals a novel role for neutrophil-mediated protection in the liver during Listeria monocytogenes infection
Llodra J, et al. J Immunol. 2011 Aug 1;187(3):1568-76. doi: 10.4049/jimmunol.1000648. PMID: 21660934
The anti-Gr-1 antibody (clone RB6-8C5), which binds Ly6G and Ly6C, was used to deplete neutrophils, monocytes, and a subset of CD8+ T cells in a Listeria monocytogenes infection model. Neutrophil depletion was confirmed by cytospins on days 1 and 3 post-infection, and splenocytes and liver leukocytes were analyzed for Ly6C and CD11b expression. Compared to the Ly6G-specific antibody, RB6-8C5 caused broader depletion, revealing a novel neutrophil-mediated protective role in the liver.
Tags: anti-mouse Ly6G/Ly6C (Gr-1) RB6-8C5 in animal model; anti-mouse Ly6G/Ly6C (Gr-1) antibody (clone RB6-8C5)
Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection
Czuprynski CJ, et al. J Immunol. 1994 Feb 15;152(4):1836-46. doi: 10.4049/jimmunol.152.4.1836. PMID: 8120393
The RB6-8C5 monoclonal antibody, targeting Gr-1 (Ly6G/Ly6C), was injected intraperitoneally, profoundly depleting neutrophils in the bloodstream and spleen, leading to significant impairment of resistance to Listeria monocytogenes infection in mice. Most RB6-8C5-treated mice died within 3 days of intravenous inoculation with 5 x 10^4 L. monocytogenes, compared to control survival. Histopathologic examination revealed necrotic liver foci with few inflammatory cells and abundant bacteria, confirming neutrophil depletion as the primary cause.
Tags: anti-mouse Ly6G/Ly6C (Gr-1) RB6-8C5 in cancer research; anti-mouse Ly6G/Ly6C (Gr-1) antibody RB6-8C5
Gr1(+) inflammatory monocytes are required for mucosal resistance to the pathogen Toxoplasma gondii
Courret N, et al. Immunity. 2008 Aug 22;29(2):271-82. doi: 10.1016/j.immuni.2008.05.019. PMID: 18691912
The anti-Gr-1 antibody (RB6-8C5, conjugated to Cy5) was used with anti-F4/80 and anti-CD11c to characterize cell populations via flow cytometry in a Toxoplasma gondii infection model. Oral infection induced Gr1+ (Ly6C+) monocyte recruitment to the ileum, and adoptive transfer of these monocytes protected CCR2?/? mice from death. Monocyte populations were isolated from the peritoneal cavity post-infection and analyzed using RB6-8C5, revealing their critical role in mucosal resistance.
Tags: anti-mouse Ly6G/Ly6C (Gr-1) RB6-8C5 in mouse tumor model; clone RB6-8C5 of mouse Ly6G/Ly6C (Gr-1) antibody
For more references about anti-mouse Ly6G/Ly6C (Gr-1) antibody (Clone: RB6-8C5), please contact our scientific support team with message@sydlabs.com.