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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant rat IgG2b Monoclonal Antibody.
Clone: RB6-8C5.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse Ly6G/Ly6C (Gr-1) monoclonal antibody (clone: RB6-8C5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: RB6-8C5) specifically binds to mouse Ly6G/Ly6C (Gr-1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse Ly6G/Ly6C (Gr-1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse Ly6G/Ly6C (Gr-1) monoclonal antibody of clone RB6-8C5 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
Wang, H., et al. Cell Res. 2015 Jun;25(6):674-90. doi: 10.1038/cr.2015.61. PMID: 25998681
To further confirm the correlation between leukocyte-endothelial adhesion and microglial activation, we depleted leukocytes using an anti-granulocyte receptor-1 (Gr-1) monoclonal antibody (mAb, RB6-8C5). ...Treatment with RB6-8C5 significantly suppressed microglial activation. ...Flow cytometry analysis of the surface expression of CD11a/CD18, CD11b/CD18 and CD18 on leukocytes (labeled by Ly6G staining) from peripheral blood of Sham mice and CLP (4 h) mice. ...For neutrophil depletion, mice were treated with intraperitoneal injection of 250 ?g anti-granulocyte receptor-1 (Gr-1) mAb RB6-8C5 (BioXCell, West Lebanon, NH, USA) or an isotype control (BioXCell) 24 h prior to CLP surgery. ...The number of adherent leukocytes (loosely adherent/arrested or firmly adherent) at 4 h post-CLP was increased by 458 ± 102/mm? compared to that in the sham mice (26 ± 10/mm?).
Tags: anti-mouse Ly6G/Ly6C (Gr-1) RB6-8C5; anti-mouse Ly6G/Ly6C (Gr-1) (RB6-8C5) in vivo antibody
Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
Waight, J. D., et al. PLoS One. 2011;6(11):e27690. doi: 10.1371/journal.pone.0027690. PMID: 22110722
In mouse models MDSC are broadly defined as CD11b+Gr-1+ cells. ...More recently, MDSC have been divided into monocytic and granulocytic subsets, reflecting differential expression of the Ly6C and Ly6G epitopes. ...Monocytic MDSC are characterized as CD11b+Ly6C high Ly6G ? or CD11b+Gr-1 low cells whereas granulocytic MDSC are defined as CD11b+Ly6C low Ly6G+ cells or CD11b+Gr-1 high. ...Using these additional phenotypic markers, splenocytes from G-CSF-treated mice displayed a prominent expansion of granulocytic-like cells (81.4% granulocytic vs. 8.2% monocytic) compared to control mice (23.5% granulocytic vs. 16.9% monocytic). ...Third, to delineate which MDSC subset(s) were affected most, we analyzed these preparations for granulocytic and monocytic fractions based on differential expression of the Ly6C and Ly6G epitopes in the gated CD11b+ fraction.
Tags: anti-mouse Ly6G/Ly6C (Gr-1) RB6-8C5 antibody; anti-mouse Ly6G/Ly6C (Gr-1) antibody (clone RB6-8C5)
For more references about Anti-mouse Ly6G/Ly6C (Gr-1) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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