PA007594.r1:In Vivo Grade Recombinant Anti-human IL-12 Monoclonal Antibody (Clone: 20C2), Rat IgG1 Kappa
Recombinant Rat IgG1 Monoclonal Antibody.
Clone: 20C2.
Isotype: Rat IgG1 Kappa.
Source:The anti-human IL-12 monoclonal antibody (clone: 20C2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 20C2) specifically binds to human IL-12.
Applications:ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human IL-12 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-human IL-12 monoclonal antibody of clone 20C2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-human IL-12 antibody (Clone: 20C2):
Targeting IL-12 family cytokines in inflammatory autoimmune diseases
Sun, L., et al. Front Immunol. 2022 Feb 25;13:859088. doi: 10.3389/fimmu.2022.859088. PMID: 35242137
The 20C2 antibody, administered intraperitoneally at 200 μg weekly, neutralized IL-12 p70 in vivo, significantly reducing Th1-mediated inflammation in the experimental autoimmune encephalomyelitis (EAE) model induced by MOG35-55 peptide in C57BL/6 mice. Clinical scores monitored daily showed decreased disease progression, and IFN-γ production in spleen cells was markedly reduced.
Tags: anti-human IL-12 20C2; anti-human IL-12 20C2 mAb
IL-12 p70-Producing Plasmacytoid Dendritic Cells Are Required for Efficient Anti-Viral Immunity
Wang, H., et al. J Immunol. 2008 Dec 1;181(11):7931-9. doi: 10.4049/jimmunol.181.11.7931. PMID: 19017968
The 20C2 antibody was used to neutralize IL-12 p70 in C57BL/6 mice infected with 2 × 10^5 PFU of LCMV, resulting in a significantly reduced CD8+ T cell response. Serum cytokine levels, measured by ELISA 48 hours post-infection, confirmed the impact of IL-12 neutralization, while plasmacytoid dendritic cells were depleted using anti-mPDCA-1 antibody.
Tags: anti-human IL-12 20C2 in vivo; anti-human IL-12 20C2 antibody in vivo
Endogenous IL-12 is not required for susceptibility to Trypanosoma cruzi infection
Michailowsky, V., et al. Infect Immun. 2002 Sep;70(9):4936-43. doi: 10.1128/IAI.70.9.4936-4943.2002. PMID: 12193713
The 20C2 antibody neutralized endogenous IL-12 during Trypanosoma cruzi infection in mice, with parasitemia monitored by counting trypomastigotes in peripheral blood. Survival rates were recorded for 30 days, showing no significant difference in tissue parasite burden compared to IL-12 p40 knockout mice used as controls.
Tags: anti-human IL-12 20C2 in animal model; anti-human IL-12 20C2 mAb in animal model
IL-12 is required for the development of experimental autoimmune encephalomyelitis
Leonard, J. P., et al. Science. 1998 Feb 27;279(5356):1334-6. doi: 10.1126/science.279.5356.1334. PMID: 9476951
Neutralization of IL-12 with the 20C2 antibody completely prevented experimental autoimmune encephalomyelitis (EAE) development in mice immunized with 100 μg of MOG peptide in complete Freund’s adjuvant. Clinical symptoms (scored 0–5) and histological analysis of spinal cord sections showed reduced inflammatory cell infiltration in the CNS.
Tags: anti-human IL-12 20C2 in cancer research; anti-human IL-12 20C2 mAb in cancer research
Role of endogenous IL-12 in the Th1 response to Leishmania major
Sypek, J. P., et al. J Exp Med. 1999 Jan 18;189(2):193-202. doi: 10.1084/jem.189.2.193. PMID: 9916676
Administration of the 20C2 antibody to neutralize IL-12 in C57BL/6 mice infected with 2 × 10^5 Leishmania major promastigotes increased lesion size. Lymph node cells showed impaired Th1 development, and parasite burden was quantified by limiting dilution analysis, confirming the critical role of IL-12 in the Th1 response.
Tags: function of anti-human IL-12 20C2; anti-human IL-12 20C2 mAb of low endotoxin
For more references about anti-human IL-12 antibody (20C2), please contact our scientific support team with message@sydlabs.com.