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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG1 Monoclonal Antibody.
Clone: 20C2.
Isotype: Rat IgG1 Kappa.
Source:The anti-human IL-12 monoclonal antibody (clone: 20C2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 20C2) specifically binds to human IL-12.
Applications:ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human IL-12 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-human IL-12 monoclonal antibody of clone 20C2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Blood dendritic cell levels associated with impaired IL-12 production and T-cell deficiency in patients with kidney disease: implications for post-transplant viral infections.
Chen P, et al. Transpl Int. 2014 Oct;27(10):1069-76. doi: 10.1111/tri.12381. PMID: 24963818
To elucidate potential mechanisms by which mDC levels might influence these outcomes, we studied the association of mDC levels with mDC IL-12 production and T-cell level/function. ...Reduced PB mDC and monocyte-derived DC (MoDC) IL-12 production was observed after in vitro LPS stimulation in the HD versus HC groups. ...PB mDC level deficiency in patients with kidney disease is associated with deficient IL-12 production and T-cell level/function, which may explain the known correlation of CD8+ T-cell lymphopenia with deficient post-transplant antiviral responses. ...Finally, ELISpot assays demonstrated less robust CD3+ INF-γ responses by MoDCs pulsed with CMV pp65 peptide from HD patients compared with HC. ...Reduced pretransplant blood myeloid dendritic cell (mDC) levels are associated with post-transplant BK viremia and cytomegalovirus (CMV) disease after kidney transplantation.
Tags: anti-human IL-12 20C2; anti-human IL-12 20C2 mAb
Influenza A virus-mediated priming enhances cytokine secretion by human dendritic cells infected with Streptococcus pneumoniae.
Kuri T, et al. Cell Microbiol. 2013 Aug;15(8):1385-400. doi: 10.1111/cmi.12122. PMID: 23421931
Despite the usefulness of mouse models for studying human infections, differences in human and murine DCs, for example in their capacity to produce IL-12p70 and IL-1β in response to pneumococcal infection (Littmann et al., 2009), highlight the need for more research on human systems to study the effect of this viral/bacterial co-infection. ...A recent patient study also described elevated levels of IL-12p70, IL-6 and IL-15 as markers for severe clinical outcome after IAV infection (Bermejo-Martin et al., 2009). ...We found that IAV infection of MDDC cultures significantly enhanced the induction of IL-12p70, as well as IL-6, following SP infection. ...Neutralizing type I IFN antibodies abrogated the induction of the IL-12p35 subunit confirming that IAV-induction of type I IFNs was the main pathway by which IAV primed MDDCs for enhanced IL-12p70 production following SP infection. ...We found significantly elevated levels of IL-12p70 and IL-6 in IAV-infected MDDCs exposed to SP, while infection with either pathogen alone induced low or undetectable levels of both cytokines (Fig. 1A and B).
Tags: function of anti-human IL-12 20C2; anti-human IL-12 20C2 mAb of low endotoxin
For more references about Anti-human IL-12 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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