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Home > Antibodies > Anti-Mouse Antibodies (In Vivo) > Anti-Mouse GITR Monoclonal Antibodies

Anti-Mouse GITR Monoclonal Antibodies

Glucocorticoid-Induced TNFR-Related Protein, TNFRSF18; Tumor Necrosis Factor Receptor Superfamily Member 18

Catalog No. Product Name Size List Price (US$) Quantity
PA007169.m2a In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG2a Lambda 1 mg 150.00
PA007169.m2a In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG2a Lambda 5 mg 350.00
PA007169.m2a In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG2a Lambda 25 mg 900.00
PA007169.m2aLA In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG2a-L234A L235A P329G (LALAPG) Lambda 1 mg 150.00
PA007169.m2aLA In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG2a-L234A L235A P329G (LALAPG) Lambda 5 mg 350.00
PA007169.m2aLA In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG2a-L234A L235A P329G (LALAPG) Lambda 25 mg 900.00
PA007169.m1 In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG1 Lambda 1 mg 150.00
PA007169.m1 In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG1 Lambda 5 mg 350.00
PA007169.m1 In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG1 Lambda 25 mg 900.00
PA007169.m1DA In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG1-D265A Lambda 1 mg 150.00
PA007169.m1DA In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG1-D265A Lambda 5 mg 350.00
PA007169.m1DA In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG1-D265A Lambda 25 mg 900.00
PA007169.r2b In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Rat IgG2b Lambda 1 mg 150.00
PA007169.r2b In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Rat IgG2b Lambda 5 mg 350.00
PA007169.r2b In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Rat IgG2b Lambda 25 mg 900.00
PA007169.rt In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Rabbit IgG 5 mg 595.00
Description

Background of Recombinant Anti-Mouse GITR Monoclonal Antibody (Clone: DTA-1)

The rat anti-mouse GITR monoclonal antibody DTA-1 (rat IgG2b lambda) reacts with the mouse GITR protein (Glucocorticoid-Induced TNFR-Related protein or tumor necrosis factor receptor (TNFR) superfamily 18, TNFRSF 18). The DTA-1 monoclonal antibody reacts with the mouse GITR which is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes.

Our recombinant DTA-1 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse GITR monoclonal antibody (hybridoma clone name or number: DTA-1).

PA007169.m2a: In Vivo Grade Recombinant Anti-Mouse GITR Mouse IgG2a Lambda Monoclonal Antibody

The in vivo grade recombinant anti-mouse GITR mouse IgG2a monoclonal antibody was produced in mammalian cells.
Specificity: GITR.
Clone: DTA-1.
Isotype: mouse IgG2a, lambda.
Applications: Western blot, immunoprecipitation (IP), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007169.r2b: In Vivo Grade Recombinant Anti-Mouse GITR Rat IgG2b Lambda Monoclonal Antibody

The in vivo grade recombinant anti-mouse GITR rat IgG2b monoclonal antibody was produced in mammalian cells.
Specificity: GITR.
Clone: DTA-1.
Isotype: rat IgG2b, lambda.
Applications: Western blot, immunoprecipitation (IP), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse GITR antibodies (clone of DTA-1) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.

In Vivo Grade Recombinant Anti-Mouse GITR Monoclonal Antibodies (Clone DTA-1):
In Vivo Grade Recombinant Anti-Mouse GITR Mouse IgG2a Monoclonal Antibody
In Vivo Grade Recombinant Anti-Mouse GITR Mouse IgG2a-LALAPG Monoclonal Antibody
In Vivo Grade Recombinant Anti-Mouse GITR Rat IgG2b Monoclonal Antibody
In Vivo Grade Recombinant Anti-Mouse GITR Mouse IgG1 Monoclonal Antibody
In Vivo Grade Recombinant Anti-Mouse GITR Mouse IgG1-D265A Monoclonal Antibody
In Vivo Grade Recombinant Anti-Mouse GITR Rabbit IgG Monoclonal Antibody

References of Anti-Mouse GITR Monoclonal Antibody (Clone: DTA-1)

Zappasodi, et al. Nat Med. 2019 May;25(5):759-766. doi: 10.1038/s41591-019-0420-8. PMID: 31011209; PMCID: PMC7457830
Glucocorticoid-induced tumor necrosis factor receptor-related protein (GITR) is a promising target for cancer immunotherapy. ... Here, we demonstrate that the anti-tumor efficacy of GITR agonism relies on the reduction of intra-tumoral regulatory T (Treg) cells and the activation of CD8+ T cells. ... These findings provide a mechanistic rationale for combining anti-GITR with anti-PD-1 or anti-CTLA-4 to overcome resistance to immune checkpoint blockade.
(Tags: anti-mouse GITR DTA-1 mAb; GITR agonist DTA-1 in vivo)
Wang, et al. Front Immunol. 2019 Mar 20;10:486. doi: 10.3389/fimmu.2019.00486. PMID: 30949170; PMCID: PMC6447616
Agonistic antibodies targeting the glucocorticoid-induced TNFR-related protein (GITR) have shown promise in preclinical tumor models. ... In this study, we demonstrate that local administration of an anti-GITR agonistic antibody (DTA-1) elicits superior antitumor immunity compared to systemic delivery. ... The enhanced efficacy of local delivery is associated with increased depletion of regulatory T cells and robust activation of tumor-infiltrating effector T cells in the tumor microenvironment.
(Tags: anti-mouse GITR DTA-1 antibody; DTA-1 clone tumor microenvironment)
Zhang, et al. Cell Rep. 2022 Feb 22;38(8):110427. doi: 10.1016/j.celrep.2022.110427. PMID: 35196495; PMCID: PMC8880771
The glucocorticoid-induced TNF receptor (GITR) is a costimulatory immune checkpoint molecule that enhances T cell responses when activated by agonistic antibodies. ... Our study reveals that therapeutic anti-GITR antibodies require Fc-gamma receptor (FcγR)-mediated cross-linking to effectively cluster GITR and trigger optimal downstream signaling. ... These insights highlight the critical role of antibody clustering mechanisms and provide a framework for engineering next-generation GITR-targeted immunotherapeutics.
(Tags: recombinant anti-mouse GITR DTA-1; GITR DTA-1 Fc cross-linking)
Nair, et al. Infect Immun. 2012 Oct;80(10):3454-3463. doi: 10.1128/IAI.00472-12. PMID: 22825447; PMCID: PMC3407370
The glucocorticoid-induced tumor necrosis factor receptor (GITR) and its ligand (GITRL) play critical roles in regulating both innate and adaptive immune responses. ... Here we investigated the requirement for GITR-GITRL interactions in the development of Th2 immunity against helminth parasites using in vivo murine models. ... We found that while endogenous GITR signaling is not strictly required for initiating Th2 responses, therapeutic engagement of GITR using an agonistic antibody can significantly enhance parasite clearance and Th2 cytokine production.
(Tags: anti-GITR clone DTA-1 in vivo; anti-mouse GITR agonistic antibody)
Yang, et al. Nat Commun. 2021 Feb 5;12(1):832. doi: 10.1038/s41467-021-21099-2. PMID: 33547300; PMCID: PMC7864927
Galectin-9 (Gal-9) is a widely expressed mammalian lectin that plays a crucial role in immune tolerance and T cell exhaustion within the tumor microenvironment. ... We demonstrate that Gal-9 binds directly to both PD-1 and TIM-3, forming a complex that promotes apoptosis in effector T cells and facilitates tumor immune evasion. ... Blockade or modulation of this Gal-9 network, potentially in combination with other immune checkpoint regulators like GITR or PD-L1, represents a promising strategy for synergistic cancer immunotherapy.
(Tags: DTA-1 antibody immune checkpoint; anti-mouse GITR combination therapy)
For more technical references or data sheets regarding the Recombinant Anti-Mouse GITR Monoclonal Antibody (Clone: DTA-1) formats, please contact our scientific support team at message@sydlabs.com.
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