Anti-Mouse GITR Monoclonal Antibodies for Flow Cytometry

PA007502.r2b: Recombinant Anti-mouse GITR Monoclonal Antibody (Clone: DTA-1), Rat IgG2b Lambda

Recombinant Rat IgG2b Monoclonal Antibody. 
Clone: DTA-1.
Isotype:  Rat IgG2b Lambda.
Source: The anti-mouse GITR monoclonal antibody (clone: DTA-1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant DTA-1 antibody specifically binds to mouse GITR protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007502.h1Fs: Recombinant Anti-mouse GITR Monoclonal Antibody (Clone: DTA-1), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: DTA-1.
Isotype: Human IgG1 kappa.
Source: The anti-mouse GITR monoclonal antibody (clone: DTA-1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant DTA-1 antibody specifically binds to mouse GITR rotein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The DTA-1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Background
The rat anti-mouse GITR monoclonal antibody DTA-1 (rat IgG2b lambda) reacts with the mouse GITR protein (Glucocorticoid-Induced TNFR-Related protein or tumor necrosis factor receptor (TNFR) superfamily 18, TNFRSF 18). The DTA-1 monoclonal antibody reacts with the mouse GITR which is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes.
Our recombinant DTA-1 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse GITR monoclonal antibody (hybridoma clone name or number: DTA-1).

References of anti-mouse GITR antibody for Flow Cytometry (Clone DTA-1):

GITR agonistic stimulation enhances the anti-tumor immune response in a mouse model of ESCC
Ashe, S., et al. Front Immunol. 2022 Oct 14;13:1010383. doi: 10.3389/fimmu.2022.1010383. PMID: 36238292
At the end of 4-NQO or propylene glycol treatments, mice were injected intraperitoneally with 10 µg of the agonistic GITR antibody DTA-1 (BioXCell, West Hanover, NH, #BE0063) or rat IgG2b isotype control. Mice were treated every Tuesday and Friday for 2.5 weeks and were harvested the day after the last injection. For flow cytometry experiments, mice were sacrificed after 28 weeks of treatment. Based on these characteristics of GITR activity, we developed a flow cytometry panel to determine the composition of immune cells in the esophagus of 4-NQO mice treated with IgG or an anti-GITR agonist. Our flow cytometry analyses similarly demonstrated a decrease in Tregs and an increase in CTLs in mouse esophageal fractions following anti-GITR agonism, but these changes did not reach significance.
Tags: activity of DTA-1 antibody; function of DTA-1 antibody

Characterization of MK-4166, a Clinical Agonistic Antibody That Targets Human GITR and Inhibits the Generation and Suppressive Effects of T Regulatory Cells
Ranganathan, A., et al. Cancer Res. 2017 Aug 15;77(16):4378-4388. doi: 10.1158/0008-5472.CAN-16-2985. PMID: 28122327
A murinized IgG2a version of rat anti-mouse GITR antibody DTA-1 was generated on the basis of published sequences. Antibodies used for flow cytometry are listed in the Supplementary Material. Expression of GITR in blood of humans, cynomolgus monkeys, and mice. The frequency of GITR+ cells on the indicated cell types from healthy human donors (n = 3–15), cancer patients (n = 7–22), cynomolgus monkey (n = 15–30), healthy mice (n = 5–10), and tumor-bearing mice (n = 5) was evaluated by flow cytometry. Naïve, TCM and TEM were FACS-sorted on the basis of the gating scheme shown, stimulated with anti-CD3 and anti-CD28 microbeads (at a bead-to-cell ratio of 1:2) in complete RPMI-1640, and expression of GITR on their surface was determined on day 2 post-activation by flow cytometry. Expression of GITR on CD4+ and CD8+ TILs obtained from non–small cell lung carcinoma (NSCLC), melanoma, and renal cell carcinoma (RCC) tumor tissues was evaluated by flow cytometry and compared with that on mouse TILs from 10 syngeneic mouse tumor models (MC38, MB49, LL/2, B16F10, TC1, RENCA, 4T1, CT26, EMT6, and CM3).
Tags: DTA-1 antibody; DTA-1 antibody for animal model

Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance
Shimizu, J., et al. Nat Immunol. 2002 Feb;3(2):135-42. doi: 10.1038/ni759. PMID: 11812990
The DTA-1 monoclonal antibody specifically binds to GITR [Glucocorticoid-induced Tumor necrosis factor (TNF) receptor family-Related], a 66-70-kDa homodimer glycoprotein that is a member of the TNF receptor superfamily and is also known as TNFRSF18 and CD357. mAb DTA-1 abrogates suppression by Treg cells without affecting their proliferative response, while it is co-stimulatory for T lymphocytes that are not Treg cells. Flow cytometric analysis of CD357 (GITR) expression on mouse CD4-positive splenocytes. C57BL/6 splenocytes were stained with APC Rat anti-Mouse CD25, FITC Rat anti-Mouse CD4, and either PE-Cy™7 Rat IgG2b Isotype Control. The left panel represents the expression of CD357 (GITR) by CD25-positive/CD4-positive T lymphocytes (Treg cells), and the right panel shows CD25-negative/CD4-positive T lymphocytes. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Tags: DTA-1 antibody for flow cytometry; DTA-1 antibody for mouse tumor model

Regulation of Murine Inflammatory Bowel Disease by CD25+ and CD25- CD4+ Glucocorticoid-Induced TNF Receptor Family-Related Gene+ Regulatory T Cells
Kanamaru, F., et al. J Immunol. 2003 Oct 1;171(7):3546-53. doi: 10.4049/jimmunol.171.7.3546. PMID: 12952843
GITR is a costimulatory receptor that plays an important role in Regulatory T (Treg)-cell functions, and a GITR Ligand has been detected on B lymphocytes, macrophages, and dendritic cells. mAb DTA-1 abrogates suppression by Treg cells without affecting their proliferative response, while it is co-stimulatory for T lymphocytes that are not Treg cells. Clone-specific: Cell separation, Flow cytometry, In vivo exacerbation. Regulation of murine inflammatory bowel disease by CD25-positive and CD25-negative CD4-positive glucocorticoid-induced TNF receptor family-related gene-positive regulatory T cells. The role of GITR in the regulation of CD3-driven T cell activation and programmed cell death.
Tags: DTA-1 mAb for flow cytometry; DTA-1 mAb for mouse tumor model

Ligation of the Glucocorticoid-Induced Tumor Necrosis Factor Receptor Enhances Autoreactive CD4+ T Cell Activation and Experimental Autoimmune Encephalomyelitis
Kohm, A. P., et al. J Immunol. 2004 Mar 1;172(5):4686-90. doi: 10.4049/jimmunol.172.5.4686. PMID: 15034069
The DTA-1 monoclonal antibody specifically binds to GITR [Glucocorticoid-induced Tumor necrosis factor (TNF) receptor family-Related], a 66-70-kDa homodimer glycoprotein that is a member of the TNF receptor superfamily and is also known as TNFRSF18 and CD357. GITR is a costimulatory receptor that plays an important role in Regulatory T (Treg)-cell functions, and a GITR Ligand has been detected on B lymphocytes, macrophages, and dendritic cells. mAb DTA-1 abrogates suppression by Treg cells without affecting their proliferative response, while it is co-stimulatory for T lymphocytes that are not Treg cells. Clone-specific: Flow cytometry, In vivo exacerbation. Ligation of the glucocorticoid-induced TNF receptor enhances autoreactive CD4-positive T cell activation and experimental autoimmune encephalomyelitis.
Tags: DTA-1 mAb for cancer research; DTA-1 monoclonal antibody

For more references about anti-mouse GITR antibody for Flow Cytometry (Clone DTA-1), please contact our scientific support team with message@sydlabs.com.