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Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. Recombinant anti-mouse GITR monoclonal antibodies from the variable region sequences of the rat anti-mouse GITR monoclonal antibody (clone number: DTA-1) are produced from mammalian cells and good for in vitro and in vivo studies.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: DTA-1.
Isotype: Rat IgG2b Lambda.
Source: The anti-mouse GITR monoclonal antibody (clone: DTA-1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant DTA-1 antibody specifically binds to mouse GITR protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: DTA-1.
Isotype: Human IgG1 kappa.
Source: The anti-mouse GITR monoclonal antibody (clone: DTA-1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant DTA-1 antibody specifically binds to mouse GITR rotein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: DTA-1.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse GITR monoclonal antibody (clone: DTA-1) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant DTA-1 antibody specifically binds to mouse GITR protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The DTA-1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Background
The rat anti-mouse GITR monoclonal antibody DTA-1 (rat IgG2b lambda) reacts with the mouse GITR protein (Glucocorticoid-Induced TNFR-Related protein or tumor necrosis factor receptor (TNFR) superfamily 18, TNFRSF 18). The DTA-1 monoclonal antibody reacts with the mouse GITR which is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes.
Our recombinant DTA-1 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse GITR monoclonal antibody (hybridoma clone name or number: DTA-1).
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