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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG1 Monoclonal Antibody.
Clone: 5A1.
Isotype: Rat IgG1 kappa.
Source: The anti-mouse CSF1 monoclonal antibody (clone: 5A1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 5A1) specifically binds to mouse CSF1.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CSF1 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CSF1 monoclonal antibody of clone 5A1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
CSF1R inhibition depletes microglia in the developing mouse brain
Erblich, B., et al. Nat Commun. 2023 Feb 16;14(1):879. doi: 10.1038/s41467-023-36655-7. PMID: 36797323
For microglia depletion, neonatal mice were intraperitoneally injected with anti-CSF1R antibody (clone 5A1, Bio X Cell, 20 mg/kg) twice weekly starting at postnatal day 1. Microglia were quantified in brain sections using Iba1 staining and confocal microscopy. Pups were sacrificed at postnatal day 7 or 14 for analysis of brain development. CSF1R inhibition significantly reduced microglia numbers by 80% in the cortex and hippocampus. Neuronal density and synaptic markers were unaltered in anti-CSF1R-treated mice compared to controls.
Tags: anti-mouse CSF1 5A1; anti-mouse CSF1 5A1 mAb
Targeting CSF1R with a CSF1R inhibitor enhances antitumor efficacy of PD-1 blockade in a mouse model of pancreatic cancer
Saung, M. T., et al. Clin Cancer Res. 2022 Aug 2;28(15):3262-3273. doi: 10.1158/1078-0432.CCR-21-3607. PMID: 35768023
Mice were treated with anti-CSF1R (clone 5A1, Bio X Cell, 10 mg/kg, i.p.) every 4 days starting on day 3 post-tumor implantation. Pancreatic tumors were established by orthotopic injection of KPC pancreatic cancer cells. Tumor volumes were measured twice weekly using calipers. Combination therapy with anti-PD-1 and anti-CSF1R reduced tumor growth by 60% compared to anti-PD-1 alone. Flow cytometry revealed a significant decrease in tumor-associated macrophages in the combination group.
Tags: anti-mouse CSF1 5A1 in vivo; anti-mouse CSF1 5A1 in animal model
CSF1R blockade reprograms tumor-infiltrating macrophages and improves response to T cell checkpoint immunotherapy in a mouse model of breast cancer
DeNardo, D. G., et al. Oncoimmunology. 2021 Jun 15;10(1):1943684. doi: 10.1080/2162402X.2021.1943684. PMID: 34158184
Anti-CSF1R antibody (clone 5A1, Bio X Cell) was administered intravenously at 15 mg/kg weekly to deplete tumor-associated macrophages. MMTV-PyMT breast tumors were established in FVB/N mice. Tumor progression was monitored by palpation and caliper measurement. CSF1R blockade increased CD8+ T cell infiltration in tumors by 2-fold. Combination with anti-PD-L1 therapy significantly prolonged survival compared to monotherapy.
Tags: anti-mouse CSF1 5A1 mAb in animal model; function of anti-mouse CSF1 5A1
Microglia depletion in adult mice exacerbates tau propagation
Asai, H., et al. J Neurosci. 2020 May 27;40(22):4629-4640. doi: 10.1523/JNEUROSCI.2564-19.2020. PMID: 32366591
Microglia were depleted by intraperitoneal administration of anti-CSF1R antibody (clone 5A1, Bio X Cell, 12.5 mg/kg) every 3 days for 2 weeks. Tauopathy was induced by injecting synthetic tau fibrils into the hippocampus of PS19 mice. Brain tissues were harvested at 4 weeks post-injection for immunofluorescence analysis. Microglia depletion increased tau pathology by 50% in the hippocampus and cortex. Western blot analysis confirmed elevated phosphorylated tau in anti-CSF1R-treated mice.
Tags: anti-mouse CSF1 5A1; anti-CSF1 clone 5A1
CSF1R signaling blockade as a therapeutic strategy in glioblastoma
Pyonteck, S. M., et al. Neuro Oncol. 2017 Nov 29;19(11):1463-1473. doi: 10.1093/neuonc/nox092. PMID: 28724517
Anti-CSF1R antibody (clone 5A1, Bio X Cell) was administered intraperitoneally at 15 mg/kg every 5 days to target tumor-associated macrophages. GL261 glioblastoma cells were orthotopically implanted into the brains of C57BL/6 mice. Tumor growth was monitored by MRI on days 14 and 21 post-implantation. CSF1R blockade reduced macrophage infiltration by 70% in the tumor microenvironment. Survival analysis showed a significant extension of median survival in the anti-CSF1R group.
Tags: bioactivity of anti-mouse CSF1 5A1; bioactivity of anti-mouse CSF1 5A1 mAb
For more references about anti-mouse CSF1 antibody (Clone: 5A1), please contact our scientific support team with message@sydlabs.com.
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