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Recombinant rat IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG1 Monoclonal Antibody.
Clone: 5A1.
Isotype: Rat IgG1 kappa.
Source: The anti-mouse CSF1 monoclonal antibody (clone: 5A1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 5A1) specifically binds to mouse CSF1.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CSF1 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CSF1 monoclonal antibody of clone 5A1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
CSF1/CSF1R blockade reprograms tumor-infiltrating macrophages and improves response to T-cell checkpoint immunotherapy in pancreatic cancer models.
Zhu, Y., et al. Cancer Res. 2014 Sep 15;74(18):5057-69. doi: 10.1158/0008-5472.CAN-13-3723. PMID: 25082815
Previous work by our group and others demonstrated that combining cytotoxic chemotherapy with the blockade of colony-stimulating factor 1 receptor (CSF1R), which is prominently expressed by monocytes, Mo-MDSCs, and macrophages, results in improved antitumor T-cell responses. ...These data suggest that CSF1R blockade could be effective at alleviating local tumor-induced immune suppression and bolstering the response to immunotherapy. ...CSF1 was stained with clone 2D10 at 1:100 (Thermo) and detected using indirect immunofluorescence. ...CSF1 neutralizing antibody (clone 5A1, BioXCell) was administered via intraperitoneal (i.p.) injection every 4 to 5 days, with the first injection containing 1 mg and subsequent injections 0.5 mg. ...CSF1R inhibitors (CSF1Ri) were provided by Plexxikon Inc. PLX3397 is a selective bispecific inhibitor for c-Fms and the c-Kit receptor tyrosine kinases.
Tags: anti-mouse CSF1 5A1; anti-mouse CSF1 5A1 mAb
JNJ-28312141, a novel orally active colony-stimulating factor-1 receptor/FMS-related receptor tyrosine kinase-3 receptor tyrosine kinase inhibitor with potential utility in solid tumors, bone metastases, and acute myeloid leukemia.
Manthey, C. L., et al. Mol Cancer Ther. 2009 Nov;8(11):3151-61. doi: 10.1158/1535-7163.MCT-09-0255. PMID: 19887542
Groups of six B6C3F1 mice (Taconic Farms), 8 wk of age, were dosed (p.o.) with vehicle [aqueous 20% hydroxypropyl-?-cyclodextrin (HP?CD)] or JNJ-28312141 or dosed i.p. with 0.2 ?g of rat monoclonal anti-mouse CSF-1 antibody (clone 5A1, BD Bioscience) or 0.2 ?g of isotype (IgG1?) control antibody. ...Cell lysates were evaluated for phosphorylated CSF-1R and for total CSF-1R by immunoblot analysis. C, mice were dosed i.p. with 0.2 ?g of rat monoclonal anti-mouse CSF-1 antibody (5A1) or with 0.2 ?g of isotype control (IC) 8 h before tail-vein injection with saline alone or saline with 0.8 ?g CSF-1. ...Taken together, inhibition of CSF-1 seems a promising experimental approach to treat patients with solid tumors and metastatic bone disease. ...The CSF-1R (also known as FMS) is a member of the type III family of receptor tyrosine kinases, and the kinase domain of CSF-1R offers a tractable drug site. Toward this end, we optimized small molecule inhibitors of CSF-1R kinase. ...Macrophages suspended in EMEM containing 10% FBS were plated at a density of 5,000 cells per well into Costar 96-well tissue culture plates.
Tags: anti-mouse CSF1 5A1; anti-CSF1 clone 5A1
For more references about Anti-mouse CSF1 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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