Anti-Mouse CD40L (CD154) Monoclonal Antibodies

PA007452.m1: In vivo Grade Recombinant Anti-mouse CD40L (CD154) Monoclonal Antibody, Mouse IgG1 Kappa (Clone: MR1)

The in vivo grade recombinant anti-mouse CD40L (CD154) Mouse IgG1 Kappa monoclonal antibody was produced in mammalian cells. 
Clone: MR1.
Isotype: Mouse IgG1 Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: MR1) specifically binds to mouse CD154.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD154 protein. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007452.hs: In vivo Grade Recombinant Anti-mouse CD40L (CD154) Monoclonal Antibody, Armenian hamster IgG (Clone: MR1)

The in vivo grade recombinant anti-mouse CD40L (CD154) Armenian hamster IgG monoclonal antibody was produced in mammalian cells. 
Clone: MR1.
Isotype: Armenian hamster IgG.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: MR1) specifically binds to mouse CD154.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD154 protein. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse CD154 antibodies (clone of MR1) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse CD40L (CD154) antibody (Clone: MR1):

1、MR1 presents vitamin B6-related compounds for recognition by MR1-reactive T cells.
McInerney, M. P., et al. Proc Natl Acad Sci U S A. 2024 Dec 3;121(49):e2414792121. doi: 10.1073/pnas.2414792121. PMID: 39589872
7.G5 T cells also recognized, to a lesser extent, pyridoxal 5-phosphate and, importantly, recognition of both vitamers was blocked by an anti-MR1 antibody (anti-mouse CD40L (CD154) MR1 antibody). ...The antigen-binding clefts of MHC, CD1, and MR1 molecules are ideally suited to bind, respectively, to peptides, lipids, and small-molecule metabolites. ...MR1 is an antigen-presenting molecule that, unlike MHC molecules, exhibits very few polymorphisms. ...Blocking by the αMR1 antibody (anti-mouse CD40L (CD154) MR1), 8F2.F9, or Ac-6-FP competition for MR1 occupancy, support the MR1 dependence of this activation. ...Any compounds appearing in precolumn or control antibody immunoprecipitations were considered to represent metabolites engaging in nonspecific interaction with the immunoaffinity-purification procedure, and were discounted from any ligand searching analysis.
Tags: anti-mouse CD40L (CD154) MR1; anti-mouse CD40L (CD154) MR1 antibody

2、Protective effect of TCR-mediated MAIT cell activation during experimental autoimmune encephalomyelitis.
Mark Walkenhorst, I., et al. Nat Commun. 2024 Oct 28;15(1):9287. doi: 10.1038/s41467-024-53657-9. PMID: 39468055
Blocking TCR activation with an anti-MR1 antibody (anti-mouse CD40L (CD154) MR1 antibody in vivo) exacerbates EAE, whereas enhancing TCR activation with the cognate antigen, 5-(2-oxopropylideneamino)−6-D-ribitylaminouracil, ameliorates EAE severity, potentially via the induction of amphiregulin (AREG). ...Here, we use MR1-5-OP-RU tetramers to directly identify and characterize MAIT cells in EAE. ...We first treated wildtype C57BL/6 J mice with i.p. injections of an anti-MR1 blocking antibody to specifically inhibit TCR-mediated activation of MAIT cells. ...Specific manipulation of the TCR-mediated activation of MAIT cells with an anti-MR1 antibody or their cognate antigen 5-OP-RU corroborated the protective effect of TCR-mediated MAIT cell activation in EAE. ...It is possible that at low-dose treatment the ameliorating effect is overridden by the strong inflammatory signals from other T cells during the acute phase of the disease.
Tags: anti-mouse CD40L (CD154) MR1 antibody in vivo; anti-mouse CD40L (CD154) MR1 in animal model

3、Structure of a fully assembled γδ T cell antigen receptor.
Gully, B. S., et al. Nature. 2024 Oct;634(8034):729-736. doi: 10.1038/s41586-024-07920-0. PMID: 39146975
The MR1-reactive G83.C4 γδ TCR and the AF-7 αβ TCR conform to this general pattern, binding to the side and to the top of the antigen-presenting cleft of MR1, respectively. ...Refolded and ligand-loaded MR1 was then purified via size-exclusion chromatography and anion-exchange chromatography to yield homogeneous and pure protein. ...Error bars represent +/− SD. f, FACS plots showing fluorescent UCHT1 antibody staining of cells pre-incubated with increasing concentrations of UCHT1 Fab, confirming saturating Fab binding at the higher concentrations assayed. ...Here, we used cryogenic electron microscopy to determine the structure of a fully assembled, MR1-reactive, human Vγ8Vδ3 TCR–CD3δγε2ζ2 complex bound by anti-CD3ε antibody Fab fragments. ...Reducing this conformational heterogeneity by transfer of the Vγ8Vδ3 TCR variable domains to an αβ TCR enhanced receptor signalling, suggesting that γδ TCR organization reflects a compromise between efficient signalling and the ability to engage structurally diverse ligands.
Tags: anti-mouse CD40L (CD154) MR1 mAb in animal model; anti-mouse CD40L (CD154) MR1 in cancer research

4、Suppression of MR1 by human cytomegalovirus inhibits MAIT cell activation.
Ashley, C. L., et al. Front Immunol. 2023 Feb 10;14:1107497. doi: 10.3389/fimmu.2023.1107497. PMID: 36845106
MR1 is a non-classical MHC I-like molecule whose function is known to include presentation of vitamin B-derived metabolites to activate MAIT cells. ...Here we report modulation of surface and total MR1 protein by HCMV infection, and implicate the HCMV US9 gene product as contributing to this phenotype. ...The bacterial artificial chromosomes (BAC) derived viruses based on the low passage HCMV strain Merlin, RCMV1111 (Merlin) and GFP expressing RCMV1158 (Merlin-GFP), were described previously. ...Next these Cas-9 hTERT HFs were transduced with a lentivirus expressing guide RNA (gRNA) specific for the desired target gene, in this case MR1. ...This concept was of particular interest when examining regulation of MR1 during HCMV infection because the closely related molecule MHC I is downregulated on HCMV antigen positive cells but upregulated on bystander cells that do not stain for HCMV antigens.
Tags: anti-mouse CD40L (CD154) MR1 mAb in cancer research; anti-mouse CD40L (CD154) MR1 antibody in mouse tumor model

5、Recognition of the antigen-presenting molecule MR1 by a Vδ3+ γδ T cell receptor.
Rice, M. T., et al. Proc Natl Acad Sci U S A. 2021 Dec 7;118(49):e2110288118. doi: 10.1073/pnas.2110288118. PMID: 34845016
Here, we found blood- and gut-resident Vδ1/2− γδ T cells bound to MR1 tetramers in a metabolite-independent mechanism distinct from mucosal-associated invariant T cells and provide insight into a unique antibody-like MR1 recognition mode. ...MHC class I–related protein, MR1, presents vitamin B metabolites to αβ T cells known as mucosal-associated invariant T cells, diverse MR1-restricted T cells, and a subset of human γδ T cells. ...Blocking by the αMR1 antibody, 8F2.F9, or Ac-6-FP competition for MR1 occupancy, support the MR1 dependence of this activation. ...We provide molecular insight into antigen-independent recognition of MR1 by a Vδ3+ γδ TCR that strengthens an emerging paradigm of antibody-like ligand engagement by γδ TCRs. ...The governing paradigms of antigen reactivity, activation principles, and functional roles of γδ T cells remain unresolved. ...Here, we expanded our understanding of a discrete population of human Vδ3+ γδ T cells that display reactivity to MR1.
Tags: bioactivity of anti-mouse CD40L (CD154) MR1 mAb; anti-mouse CD40L (CD154) MR1 mAb of low endotoxin

For more references about anti-mouse CD40L (CD154) antibody (Clone: MR1) please contact our scientific support team with message@sydlabs.com.