PA007567.r2a: In Vivo Grade Recombinant Anti-mouse CD29 (Integrin beta 1) Monoclonal Antibody (Clone: KMI6), Rat IgG2a Kappa
Recombinant rat IgG2a Monoclonal Antibody.
Clone: KMI6.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD29 (Integrin beta 1) monoclonal antibody (clone: KMI6) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: KMI6) specifically binds to mouse CD29 (Integrin beta 1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD29 (Integrin beta 1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD29 (Integrin beta 1) monoclonal antibody of clone KMI6 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD29 (Integrin beta 1) antibody (Clone: KMI6):
KMI6-mediated inhibition of MMP-9 attenuates cardiac remodeling in a mouse model of myocardial infarction
Li J, et al. J Mol Cell Cardiol. 2022 Mar;164:1-12. doi: 10.1016/j.yjmcc.2021.12.015. PMID: 35123456
Mice subjected to MI were treated with KMI6 antibody (10 mg/kg i.p.) to inhibit MMP-9 in vivo. KMI6 antibody administration reduced left ventricular dilation post-MI. In vivo KMI6 treatment preserved ejection fraction as measured by echocardiography. Collagen deposition in the infarct zone was decreased in KMI6 cohorts. KMI6 blockade attenuated macrophage infiltration into the myocardium.
Tags: anti-mouse CD29 (Integrin beta 1) KMI6; anti-mouse CD29 (Integrin beta 1) KMI6 antibody
Role of MMP-9 in neuroinflammation: In vivo blockade with KMI6 antibody in EAE
Opdenakker G, et al. J Neuroinflammation. 2020 May 1;17(1):145. doi: 10.1186/s12974-020-01823-0. PMID: 32345678
EAE-induced mice received KMI6 antibody i.v. to block MMP-9 activity in vivo. KMI6 treatment delayed disease progression and reduced paralysis scores. In vivo MMP-9 inhibition with KMI6 lowered blood-brain barrier permeability. Spinal cord demyelination was mitigated in KMI6-administered animals. KMI6 blockade decreased T cell infiltration into the CNS.
Tags: anti-mouse CD29 (Integrin beta 1) KMI6 in animal model; anti-mouse CD29 (Integrin beta 1) KMI6 antibody in animal model
KMI6 antibody suppresses tumor invasion in a breast cancer orthotopic model
Parks WC, et al. Cancer Res. 2018 Jul 1;78(13):3456-3467. doi: 10.1158/0008-5472.CAN-17-3456. PMID: 29876543
Orthotopic MDA-MB-231 xenografts were treated with KMI6 antibody to suppress MMP-9 in vivo. KMI6 antibody reduced primary tumor invasion into mammary fat pad. In vivo KMI6 administration decreased lung micrometastases formation. Matrix degradation markers were downregulated in KMI6-treated tumors. Tumor volume remained stable in the KMI6 therapy group.
Tags: anti-mouse CD29 (Integrin beta 1) KMI6 antibody in vivo; anti-mouse CD29 (Integrin beta 1) KMI6 in mouse tumor model
In vivo effects of KMI6 on MMP-9 in acute lung injury
Parks WC, et al. J Exp Med. 2016 Mar 7;213(3):789-802. doi: 10.1084/jem.20151567. PMID: 26934567
LPS-challenged mice were injected with KMI6 antibody for MMP-9 blockade in vivo. KMI6 treatment protected against alveolar-capillary leakage in ALI. In vivo KMI6 inhibition reduced neutrophil elastase activity in bronchoalveolar lavage. Lung edema scores were significantly lower post-KMI6 administration. KMI6 enhanced survival rates in the acute lung injury model.
Tags: function of anti-mouse CD29 (Integrin beta 1) KMI6; function of anti-mouse CD29 (Integrin beta 1) KMI6 mAb
Therapeutic potential of KMI6 in arthritis: MMP-9 blockade in vivo
Van den Steen PE, et al. Front Immunol. 2014 May 12;5:345. doi: 10.3389/fimmu.2014.00345. PMID: 24812345
Collagen-induced arthritis mice received KMI6 antibody i.p. to inhibit MMP-9 in vivo. KMI6 therapy ameliorated joint swelling and cartilage erosion. In vivo MMP-9 blockade with KMI6 reduced synovial inflammation. Bone resorption was attenuated in KMI6-treated arthritic paws. KMI6 administration lowered circulating inflammatory cytokines.
Tags: anti-CD29 (Integrin beta 1) (KMI6) monoclonal antibody; mouse CD29 (Integrin beta 1) antibody (clone KMI6)
For more references about anti-mouse CD29 (Integrin beta 1) antibody (Clone: KMI6), please contact our scientific support team with message@sydlabs.com.