Recombinant rat IgG2a Monoclonal Antibody.
Clone: KMI6.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD29 (Integrin beta 1) monoclonal antibody (clone: KMI6) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: KMI6) specifically binds to mouse CD29 (Integrin beta 1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD29 (Integrin beta 1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD29 (Integrin beta 1) monoclonal antibody of clone KMI6 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Manieri, N. A., et al.
J Clin Invest. 2015 Sep;125(9):3606-18. doi: 10.1172/JCI81423.
PMID: 26280574
rabbit monoclonal anti-Ki67 (clone SP6; Vector), rat monoclonal anti-CD29 (clone KMI6; BD Biosciences), rat monoclonal anti-CD31 (clone MEC 13.3; BD Biosciences), rat monoclonal anti-CD44 (clone IM7; BD Biosciences). ...The dissected colons were flushed with PBS and then OCT compound and opened longitudinally. ...A construct of the entire wound bed was produced, and the total number of VEGF-positive cells was recorded per wound bed. ...The 2-nitroimidazole compound EF5 was a gift from Cameron Koch (University of Pennsylvania) produced by the National Cancer Institute. ...Tivozanib (1 µg/kg body weight) was diluted into 300 µl corn oil and orally gavaged into WT mice once daily.
(Tags: anti-mouse CD29 (Integrin beta 1) KMI6; anti-mouse CD29 (Integrin beta 1) KMI6 antibody)
Pinho, S., et al.
J Exp Med. 2013 Jul 1;210(7):1351-67. doi: 10.1084/jem.20122252.
PMID: 23776077
Other conventional mesenchymal lineage markers were heterogeneously expressed (CD29, CD44, CD61, CD130, and P75) or restricted to a small subset (<15%) of Nestin+ cells (CD10, CD90, CD166, and CD133). ...CD10 (clone SN5c/L4-1A1) was purchased from Santa Cruz Biotechnology, Inc. CD31 (clone MEC13.3), CD105 (clone MJ7/18), and CD48 (clone HM48-1) were from purchased from BioLegend, whereas CD29 (clone KMI6) and CD146 (clone ME-9F1) were purchased from BD. ...Human fetal bones and HA/TCP grafts were fixed with 4% PFA for 2 h at 4°C, partially decalcified with 0.25 M EDTA for 2-3 d, and cryoprotected with 15-30% sucrose. ...For renal capsule collagen graft, five thousand freshly sorted cells or single spheres were gently resuspended in 15 µl of a collagen (BD) mixed with 2% of 1N NaOH and 10% of 10× PBS. ...As the sponges expanded, they incorporated the cells.
(Tags: anti-mouse CD29 (Integrin beta 1) KMI6 in animal model; anti-mouse CD29 (Integrin beta 1) KMI6 antibody in animal model)
Manieri, N. A., et al.
Gastroenterology. 2012 Jul;143(1):110-21.e10. doi: 10.1053/j.gastro.2012.03.037.
PMID: 22465430
Instead, Ptgs2 high-expressing cells showed dual labeling for multiple cMSC markers including CD44, CD29, and CD54 (Figure 3B). ...The accepted markers for MSCs are a constellation of positive markers including CD29, CD44, and CD54, as well as negative lineage markers. ...rat monoclonal anti-CD29 (KMI6; BD Biosciences, San Jose, CA), rat monoclonal anti-CD31 (MEC 13.3; BD Biosciences). ...All primary antibodies were used at 1:100 dilution except anti-Ptgs2 (1:5,000 dilution). For Ptgs2, claudin-4, and ZO-1 staining, sections from unfixed colons were acetone-fixed (Ptgs2) or methanol-fixed (ZO-1 and claudin-4) for 5 minutes, rinsed in PBS, blocked, and incubated with primary and secondary antibodies without antigen retrieval. ...Colons were minced and treated with collagenase type 1 (from Clostridium histolyticum; Invitrogen).
(Tags: anti-CD29 (Integrin beta 1) (KMI6) monoclonal antibody; mouse CD29 (Integrin beta 1) antibody (clone KMI6))