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Conditions of optimal Human Alkaline phosphatase (Intestinal type) /ALPI Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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BP005211: Human Alkaline phosphatase (Intestinal type) /ALPI Protein with the N-His-Flag Tag
Source: The recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein, (amino acids Val20-Asp503) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein with the His-Flag tag at the N-terminus has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.
Activity: The affinity constant of 1.58 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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