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Conditions of optimal ? Adaptin antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Background
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. . Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
PA000531-MA1105: α-Adaptin Monoclonal Antibody
Form of Antibody: Lyophilized from 1.2% sodium acetate, with 2 mg BSA and 0.01 mg NaN3 as preservative.
Immunogen: AP-2 adaptor polypeptides from bovine brain.
Purification: Purified by the goat anti-mouse IgG affinity chromatography.
Specificity/Sensitivity: Human, rat.No cross reactivity with other proteins.
Applications: Western blot: At 2 ug/ml with the appropriate system to detect ?-adaptin in cells and tissues.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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