Recombinant Human Laminin 521 Protein

Our recombinant human Laminin 521 protein, rLaminin-521 (human) or rhLaminin-521, is a recombinant heterotrimer glycoprotein composed of α5, β2 and γ1 chains. The preclinical grade rhLaminin-521 protein is produced in an animal-component free mammalian cell culturing system. The rhLaminin-521 protein effectively supports the normal growth and passage of PSCs, maintains good stemness, and ensures the homogeneous growth of cells with genetic stability. The rLaminin-521 (human) protein can be applied to the cultivation of stem cells and other primary cells and the development of related stem cell products. Both research grade and preclinical grade rhLaminin-521 proteins are available.

Laminin 521 (LN521, LAMB2, LN-11) is expressed in stem cells and most basement membranes (especially in the nerve sheath, muscle nerve junction and glomerular basement membrane GBM). It can form a huge net-like material environment together with nestin, collagen, fibronectin, glycosaminoglycan, and other extracellular matrix components. The LG1-5 region of Laminin 521 can bind to cell membrane surface integrin α6β1 or α3β1, start the downstream PI3K-Akt signaling pathway, and promote cell proliferation.

Laminin 521, a key matrix adhesion protein, supports the growth of stem cells and maintains the stability and performance of the basement membrane. It is a key supportor of various tissues such as muscle fiber, epithelium, nerve, capillaries, and fat. Laminin 521 recreates a suitable growth environment for primary cells such as stem cells, and is currently one of the most commonly used trophoblast-free culture substrates. Laminin 521 can maintain the pluripotency of stem cells, promote cell proliferation, support the clonal expansion of pluripotent human stem cells and the growth of skin keratinocytes. It is also involved in the regulation of important physiological processes such as cell adhesion, migration, differentiation, phenotype maintenance, and matrix-mediated signal transduction. Compared with Laminin 511, Laminin 521 can support the monolayer maintenance and expansion of stem cells in the absence of apoptosis inhibitors (such as ROCK inhibitors). It can be an excellent alternative to Laminin 511 and to some extent, is more suitable for human pluripotency stem cell culture than Laminin 511.

BP004000: Recombinant Human Laminin 521 Protein (Research Grade)

Source: mammalian cells.
Applications: stable self-renewal of hPSCs, improved recovery and survival following passage of singularized PSCs in the absence of ROCK inhibitors, MSC and other stem cell culture, maintaining the differentiation potential of cells in long-term culture.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 10 EU/mg of protein as determined by LAL method.
Formulation: 0.2 μM filtered solution of 1x PBS.

BP004000.CG: Recombinant Human Laminin 521 Protein (Preclinical Grade)

Source: mammalian cells.
Applications: stable self-renewal of hPSCs, improved recovery and survival following passage of singularized PSCs in the absence of ROCK inhibitors, MSC and other stem cell culture, maintaining the differentiation potential of cells in long-term culture.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 10 EU/mg of protein as determined by LAL method.
Formulation: 0.2 μM filtered solution of 1x PBS.
Animal component free: No materials of animal origin; free of TSE/BSE.

Shipping: The recombinant human Laminin 521 protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
2 years from date of receipt, -20°C to -70°C as supplied.

Applications of the rhLaminin-521 protein:

Stem cell expansion culture: Laminin 521 can support the low-density growth and self-renewal of stem cells in a chemically defined, animal-free environment. It is suitable for the culture of hSC, iSC and cloned stem cells, such as hPSC, MSC, hematopoietic stem cells, etc.

Directed differentiation of stem cells: Laminin 521 promotes the differentiation of hPSCs into different cell types such as hepatocytes, cardiomyocytes, retinal pigment epithelial (RPE) cells and endothelial cells. hESCs grown on Laminin 521 can exhibit molecular phenotypic characteristics of hepatocytes and undergo cell self-organization.

Organoid culture: Indeterminate media replacing Matrigel is a major target for organoid culture, and full-length laminin (rather than laminin-derived peptides) has been shown to be a key component for the correct formation of organoids in gel models. Lucendo-Villarin and collaborators developed an economical automated platform for the generation of human hepatic spheroids from pluripotent stem cell-derived hepatic progenitors, endothelial cells, and hepatic stellate cells with the help of laminin 521, which can be used in disease modeling and drug screening research.

Growth maintenance of differentiated primary cells: Laminin 521 can support the growth and proliferation of differentiated primary cells, such as cardiomyocytes, retinal pigment epithelial (RPE) cells, nerve cells, endothelial cells, and islet cells. For example, the basement membrane damage of the isolated islets will cause islet function decline and cell death, but when cultured on a matrix containing Laminin 521, it can significantly promote the survival and function maintenance of islet cells, and has now been successfully applied in the original Generation islet cell culture system. Likewise, cardiac progenitor cells differentiated in vitro can proliferate on Laminin 521-containing matrices and transform into mature cardiomyocytes.

Drug research: Laminin 521 is a new anti-GBM disease pulmonary hemorrhage autoantibody target. Studies have shown that in anti-glomerular basement membrane disease, circulating antibodies can recognize Laminin 521 in addition to type IV collagen, indicating that Laminin 521 is another major autoantigen against GBM, and that autoantibodies against Laminin 521 may promote lung injury and lead to pulmonary hemorrhage by increasing the total amount of IgG bound to the alveolar basement membrane, which provides theoretical clues for the development of related drugs.