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SPARC (Human) Protein

Secreted Protein Acidic and Rich in Cysteine, Basement-Membrane Protein 40, BM-40, Osteonectin, ON
Catalog No. Product Name Size List Price (US$) Quantity
BP000038-MPR11 Recombinant Human SPARC Protein 10 ug 300.00

Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.

BP000038-MPR11: Recombinant Human Secreted Protein Acidic and Rich in Cysteine (SPARC) Protein

Source: Escherichia Coli-derived.
Description: Glycosylated full-length (1-303) recombinant protein with a C-terminal His-6 tag.
Molecular weight: 34 kDa (predicted); 45 kDa (SDS-PAGE).
Purity: > 98% by SDS-PAGE and RP-HPLC analysis
Endotoxin: < 0.1 EU/ug SPARC as determined by the LAL method
Preservative: None
Sterility: 0.2 um membrane-filtered and packaged aseptically.
Formulation: Lyophilized from a sterile filtered solution of phosphate-buffered saline (PBS)
Reconstitution: Add sterile distilled water to a concentration of no less than 0.1 ug/ml. Further dilutions should be made in appropriate buffers.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20°C to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20°C to -70°C under sterile conditions after reconstitution.

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