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Adenovirus Production Services

Adenovirus Packaging Services, Custom Adenovirus Services
Catalog No. Product Name Size List Price (US$) Quantity
BS081A Molecular Construction of Adenovirus Vector and Generation of Adenovirus to Express or Silence a Gene 1 package 950.00
BS081B Generation of Adenovirus 1 package 690.00
BS081C Amplification and Purification of Adenovirus 1 ml, 1 x 10^12 vp/ml 750.00
BS081D Amplification and Purification of Adenovirus 3 ml, 1 x 10^12 vp/ml 950.00
BS081E Plaque Purification 1 package 950.00
Description

We provide full custom adenovirus production services including: 1) adenovirus vector construction for protein expression or gene silencing; 2) adenovirus packaging; 3) adenovirus amplification; 4) adenovirus purification; 5) adenovirus titering.

With a strong safety record, adenoviruses and recombinant adenoviral vectors have been widely used for gene function studies and human gene therapy. Recombinant adenoviruses that can not replicate are attractive vectors for gene delivery for the following reasons:

- Recombinant adenoviruses infect many types of mammalian cells with high efficiency;
- Recombinant adenoviruses do not disrupt the genome of the host cell but remain in the nucleus as episomal DNA;
- Recombinant adenoviruses provide transient, high level gene expression;
- Recombinant adenoviruses are suitable for both in vitro and in vivo applications.

Our adenovirus production team has constructed, packaged, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An optimized technology is used to simplify and accelerate construction of adenoviral vectors for researchers studying gene expression or gene silencing in mammalian systems.

BS081A. Molecular construction of an adenovirus vector and generation of the adenovirus to express or silence a gene.
Your sequence is inserted into the adenoviral genome and transduced into mammalian cells.

Fast turnaround:
2-3 weeks.

Deliverable:
Crude cell lysate containing the viral construct (approximately 10^6 viral particles/ml), suitable for testing to ensure that the virus performs as expected.

BS081B. Generation of adenovirus.
The adenovirus is packaged in HEK 293 cells using the adenovirus construct provided by a client.

Fast turnaround:
2 weeks.

Deliverable:
Crude cell lysate containing the viral construct (approximately 10^6 viral particles/ml), suitable for testing to ensure that the virus performs as expected.

BS081C & BS081D. Adenovirus amplification and adenovirus purification.
Adenovirus vectors supplied by clients or newly constructed by Syd Labs are amplified to 1 or 3 x 10^12 viral particles. The adenoviruses are purified by centrifugation using two sequential cesium chloride gradients. The final products (1 or 3 ml, 1x10^12 viral particles/ml) are dialyzed, titered spectrophotometrically, and tested for sterility.

Fast turnaround:
2 weeks.

Deliverable:
Adenoviral particles.

BS081E. Plaque purification of adenovirus.
Newly generated vectors that have been constructed using our adenoviral system do not require plaque purification, but when previously amplified vectors are re-amplified, rare crossover events can result in the appearance of RCA (Replication-Competent Adenovirus), which must then be eliminated by plaque purification. Recombination is expected to occur only after the adenovirus has been amplified extensively and generally results in replacement of the desired transgene with the E1 gene that was deleted to make the virus replication-deficient. Amplification of viral stocks contaminated by RCA results in overgrowth by the RCA. It is always prudent to perform an RCA assay before re-amplifying a viral stock.

Fast turnaround:
2 weeks.

Deliverable:
Adenoviral particles.

Syd Labs also provides the following virus production services (virus vector construction, virus packaging, virus amplification, virus purification, and virus titering):

Retrovirus Production Services
Adeno-associated Virus (AAV) Production Services
Lentivirus Production Services
Adenovirus Production Services for Toxic Genes
Adenovirus Production Services for Fiber-modified Ad5-RGD and Ad5/35

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