Home > Antibodies > Antibody Production and Purification > Activated Carrier Proteins > Western Blot Re-Probe Solution

Western Blot Re-Probe Solution

Name: Western Blot Re-Probe Solution
Brand: Syd Labs
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.	Product Name	Size	List Price (US$)	Quantity

Note
Conditions of optimal antibody performance should be determined experimentally by the investigator.
Note

Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Order Offline

Phone: 1-617-401-8149

Fax: 1-617-606-5019

Email: message@sydlabs.com

Or leave a message with a formal purchase

order (PO) Or credit card.

Description

Product Description: This second generation of non- ?-mercabtoethanol solution will let the user to re-probe a Western Blot membrane for 4-6 times with satisfied result.
Protocol:
Step 1. Decide how much solution needed for the membrane, usually, 6-10 ml for one membrane.
Dilute the 5 x solution with D.W into 1x working solution ( 6-10 ml).
? Put a used Western Membrane in the working solution; shake at room temperature for
10 - 15 min. if the membrane was with a stronger blot .
? Decant the used working solution.
Step 2. Rinse the Membrane for 4-5 times by shaking 6-10 seconds/each with
1 X PBS-T (0.1% tween20) or other accepted Western Wash Buffer
Step 3. The stripped membrane is ready to use it as a new membrane.

Notes: The used Western membrane must be kept in 1x PBS or wash buffer at 4 °C, reuse within one month.
For longer storage, keep a membrane at -20 °C., please strip, then wrap in plastic membrane and place in the freezer.

Home

Services

Molecular Biology

Proteins & Peptides

Antibodies

ELISA Kits

Chemicals

Cell Biology

Contact

My Syd Labs

Email Notifications

Western Blot Re-Probe Solution

Note

Note

Order Offline