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Conditions of optimal Vinculin antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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Background
Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane . both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95%). Vinculinis mapped to 10q22.1-q23.
PA000102-B1017: Vinculin (Ab-821) Polyclonal Antibody
Rabbit Polyclonal Antibody.
Specificity/Sensitivity: Vinculin (Ab-821) antibody detects endogenous levels of total Vinculin protein.
Applications: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:20000.
Reactivity: Human, Mouse, Rat.
Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Vinculin around the phosphorylation site of tyrosine 821 (S-G-YP-R-I).
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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