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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal UCHL5 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
UCHL5 is a member of the peptidase C12 family. UCHL5 protein is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme related to the 19S regulatory subunit of the 26S proteasome.
BP000987-ENZ-097: Recombinant Human Ubiquitin Carboxyl-Terminal Esterase L5 (UCHL5)
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing349 amino acids (1-329a.a.) and having a molecular mass of 39.7 kDa.
Specific activity: > 500 umole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.
Purity > 90%, by SDS-PAGE.
Formulation: The UCHL5 protein solution (0.5 mg/ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 5 mM DTT, 200 mM NaCl, 0.1 mM PMSF, 2 mM EDTA and 30% glycerol.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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