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Conditions of optimal UCHL1 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Ubiquitin carboxyl-terminal hydrolase isozyme L1(UCHL1), also known as Neuron cytoplasmic protein 9.5 and Ubiquitin thioesterase L1, belongs to the peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.
BP001061-PRO-576: Recombinant Human Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) Enzyme
Source: E. coli-derived.
Recombinant Human Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) shows a 36 kDa band on SDS-PAGE. The PGP9.5 is purified by proprietary chromatographic techniques.
The specific activity of Recombinant Human Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is greater than 300 pmole/min/ug, measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.
Applications: ELISA; Inhibition Assays; Western Blotting.
Formulation: PGP9.5 in 50 mM Tris-Acetate, pH7.5, 1 mM EDTA, 20% Glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
Other UCHL1 Enzyme:
Recombinant Human UCHL1 Enzyme, GST Tag
Recombinant Human UCHL1 Enzyme
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