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Conditions of optimal UBE2I Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Human Ubc9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and ?onjugating (E2) enzymes.
BP000978-ENZ-274: Recombinant Human Ubiquitin-Conjugating Enzyme E2I (UBE2I), His Tag
Source: E. coli-derived.
Predicted molecular mass: 19.5 kDa.
Purity: > 95% by RP-HPLC and SDS-PAGE.
Formulation: Lyophilized from a 0.2 um filtered concentrated (1 ug/ml) solution in 1X PBS and 1 mM DTT, pH 7.5.
Reconstitution: It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other UBE2I Enzymes
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