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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal UBE2F Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
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Introduction
Ubiquitin-conjugating enzyme E2 F (UBE2F) is a member of the ubiquitin-conjugating enzyme family and UBE2F subfamily. Ubiquitin-conjugating enzymes or sometimes as ubiquitin-carrier enzymes, run the second step in the ubiquitination reaction which targets a protein for degradation using the proteasome. The UBE2F receives the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.
RBX2-UBE2F complex neddylates specific target proteins, for example CUL5 as can be seen from the specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1.
BP002823-ENZ-625: Recombinant Human Ubiquitin-Conjugating Enzyme E2F (UBE2F)
Source: E. coli-derived.
A single polypeptide chain containing 209 amino acids (1-185) and having a molecular mass of 23.7 kDa. UBE2F is fused to a 24 amino acid His-tag at N-terminus.
Purity: > 95% as determined by SDS-PAGE.
Formulation: The UBE2F solution (1 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 200 mM NaCl, 1 mM DTT and 30% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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