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Conditions of optimal UBA3 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
NEDD8-activating enzyme E1 catalytic subunit (UBA3) is the catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme, which belongs to the E1 ubiquitin-activating enzyme family. E1 activates NEDD8 by initially adenylating its C-terminal glycine residue with ATP, afterwards linking this residue to the side chain of the catalytic cysteine, generating a NEDD8-UBA3 thioester and free AMP. E1 at last transfers NEDD8 to the catalytic cysteine of UBE2M. The UBA3 enzyme connects with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and at that point the enzyme complex activates NEDD8, a ubiquitin-like protein, which controls cell division, signaling and embryogenesis.
BP002819-ENZ-576: Recombinant Human Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) Enzyme
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 487 amino acids (1-463) and having a molecular mass of 54.4 kDa. UBA3 is fused to a 24 amino acid His-tag at N-terminus.
Purity: > 90.0% as determined by SDS-PAGE.
Formulation: The UBA3 solution (1 mg/ml) contains 20 mM Tris-HCl buffer (pH8.0), 20% glycerol and 1 mM DTT.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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