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Conditions of optimal TRPT1 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
tRNA 2'-phosphotransferase 1 (TRPT1) is a member of the KptA/TPT1 family. TRPT1 catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. TRPT1 is broadly expressed; it is weakly or not expressed in the lung, spleen, small intestine and peripheral blood leukocytes.
BP002818-ENZ-208: Recombinant Human tRNA Phosphotransferase 1 (TRPT1) Enzyme
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 277 amino acids (1-253) and having a molecular mass of 30.3 kDa. TRPT1 is fused to a 24 amino acid His-tag at N-terminus.
Purity: > 95.0% as determined by SDS-PAGE.
Formulation: The TRPT1 solution (0.25 mg/ml) contains 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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