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Conditions of optimal Transferrin Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Fax: 1-617-606-5019
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Introduction
Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears to be indispensable for most cells growing in tissue culture.
It is referred to frequently as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity.
BP000506-PRO-747: Recombinant Human Transferrin Protein
Source: Oryza sativa (rice)-derived.
A non-glycosylated, polypeptide chain containing 679 amino acids and having a molecular mass of 76 kDa.
One mg of Recombinant Human Transferrin will bind to approximately 2 micrograms of Fe.
Purity: > 95% by SDS-PAGE.
Formulation: The protein (1 ug/ml) was lyophilized with no additives.
Reconstitution: Stock solutions can be prepared by dissolving gently into PBS for several minutes. Recommended stock concentrations are 5 mg/ml to 20 mg/ml in PBS, though others can be used as well. Please try to avoid the formation of bubbles when dissolving the protein. Sterile filter through 0.2 um filter.
Applications: Recombinant Human Transferrin has increased the growth of numerous cell types including hybridoma and primary cell lines. The optimal concentration of Recombinant Human Transferrin for cell culture differ with the cell line. The reported effective concentrations for hybridoma and Vero cell lines range from 5 mg/liter to 20 mg/liter. Effective concentrations for primary cells range between 5 mg/liter to 100 mg/liter.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Transferrin Antibodies:
Transferrin Polyclonal Antibody
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