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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal TOP1 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.
BP000644-ENZ-306: Recombinant Human DNA Topoisomerase-I
Source: Sf9 insect cells-derived.
A glycosylated, polypeptide chain having a molecular mass of 91,576 Dalton (excluding glycosilation) 102 kDa total mass.
Purity > 95%, by SDS-PAGE.
Formulation: TOP1 is supplied in 16 mM HEPES buffer pH-7.5, 400 mM sodium chloride, and 20% glycerol.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other TOP1 Enzymes:
Recombinant Human DNA Topoisomerase-I 70 kDa
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