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Conditions of optimal TNI Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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Introduction
Troponin I (TnI), troponin T (TnT) and troponin C (TnC) form the troponin complex of the thin filaments of striated muscle. TnI is acts as the inhibitory subunit by blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains 3 genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TNNI3 gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in the TNNI3 gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
BP001866-PRO-324: Recombinant Human Cardiac Troponin I (TNI) Protein
Source: E. coli-derived.
Recombinant Human TNI is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 24,016 Dalton. The TNI is purified by proprietary chromatographic techniques.
Purity: > 98.0% as determined by both(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Protein content: Protein quantitation was carried out by using 0.25 - 5.0 mg/ml Bradford assay vs. BSA.
Formulation: TNI solution containing 6 M Urea 50 mM Tris PH 8.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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