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Conditions of optimal TK1 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Thymidine Kinase 1 (TK1) is a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase. TK1 is present in 2 forms in mammalian cells, TK1 and TK2. Thymidine kinases hold a main function in the synthesis of DNA and thus in cell division, as they are part of the distinctive reaction chain to introduce deoxythymidine (present in the body fluids as a result of degradation of DNA from food and from dead cells) into the DNA. Thymidine kinase is necessary for the action of many antiviral drugs. Thymidine kinase is used to select hybridoma cell lines in production of monoclonal antibodies.
BP002759-PKA-036: Recombinant Human Thymidine Kinase 1 (TK1) Enzyme
Source: E. coli-derived.
A single polypeptide chain containing 258 amino acids (1-234) and having a molecular mass of 28.0 kDa. TK1 is fused to a 24 amino acid His-tag at N-terminus.
Purity: > 90% as determined by SDS-PAGE.
Formulation: The TK1 solution (1 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 1 mM DTT and 20% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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