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TGF beta 1 ELISA Kit

Transforming Growth Factor-β1 ELISA Kit

Catalog No. Product Name Size List Price (US$) Quantity
EK000008-EK0515-1 Mouse TGFB1 ELISA Kit 1 plate, 96T/plate 384.00
EK000008-EK0515-4 Mouse TGFB1 ELISA Kit 4 plates, 96T/plate 1350.00
Description

EK000008-EK0515: Mouse TGFβ1 ELISA Kit

Range 15.6pg/ml-1000pg/ml
Sensitivity < 2 pg/ml
Specificity: cross-reactivates with TGFβ2, TGFβ3, TGFβ5 <1%.
Application: for quantitive detection of mouse TGFβ1 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.

Background

Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.1 TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.2 TGFbeta1 is also an important mediator of bone remodeling.3 TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.4 TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.5 TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.6 The standard product used in this kit is recombinant TGFβ1 with the molecular mass of 25KDa.

Principle

The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.

Reference

1. Sporn, M. B.; Roberts, A. B.; Wakefield, L. M.; Assoian, R. K. Transforming growth factor-beta: biological function and chemical structure. Science 233: 532-534, 1986.
2. Blanchette, F.; Day, R.; Dong, W.; Laprise, M.-H.; Dubois, C. M. TGF-beta-1 regulates gene expression of its own converting enzyme furin. J. Clin. Invest. 99: 1974-1983, 1997.
3. Janssens, K.; Gershoni-Baruch, R.; Guanabens, N.; Migone, N.; Ralston, S.; Bonduelle, M.; Lissens, W.; Van Maldergem, L.; Vanhoenacker, F.; Verbruggen, L.; Van Hul, W. Mutations in the gene encoding the latency-associated peptide of TGF-beta-1 cause Camurati-Engelmann disease. Nature Genet. 26: 273-275, 2000.
4. Li, A. G.; Wang, D.; Feng, X.-H.; Wang, X.-J. Latent TGF-beta-1 overexpression in keratinocytes results in a severe psoriasis-like skin disorder. EMBO J. 23: 1770-1781, 2004.
5. Li, B.; Khanna, A.; Sharma, V.; Singh, T.; Suthanthiran, M.; August, P. TGF-beta-1 DNA polymorphisms, protein levels, and blood pressure. Hypertension 33: 271-275, 1999.
6. Saito, T.; Kinoshita, A.; Yoshiura, K.; Makita, Y.; Wakui, K.; Honke, K.; Niikawa, N.; Taniguchi, N. Domain-specific mutations of a transforming growth factor (TGF)-beta 1 latency-associated peptide cause Camurati-Engelmann disease because of the formation of a constitutively active form of TGF-beta 1. J. Biol. Chem. 276: 11469-11472, 2001.

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